Horm Metab Res 2007; 39(4): 250-255
DOI: 10.1055/s-2007-973075
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

TNF-α Alters Visfatin and Adiponectin Levels in Human Fat

J. Hector 1 , B. Schwarzloh 1 , J. Goehring 1 , T. G. Strate 2 , U. F. Hess 1 , G. Deuretzbacher 3 , N. Hansen-Algenstaedt 3 , F.-U. Beil 1 , P. Algenstaedt 1
  • 1Department of Internal Medicine III, University Medical Center Hamburg-Eppendorf, Germany
  • 2Department of General, Visceral- and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Germany
  • 3Spine Center, University Medical Center Hamburg-Eppendorf, Germany
Further Information

Publication History

received 4. 9. 2006

accepted 20. 11. 2006

Publication Date:
19 April 2007 (online)

Abstract

Adiponectin and visfatin are newly discovered adipokines that are strongly expressed in human visceral adipose tissue. To identify new regulatory mechanisms in fat, the effect of TNF-alpha (TNF) on adiponectin, on its two receptors, and on visfatin was investigated by incubating human visceral adipose tissue from patients without diabetes mellitus with TNF for 24, 48 and 72 hours. The mRNA expression of visfatin, adiponectin, and its two receptors, as well as the protein expression of adiponectin were determined. A decrease of adiponectin mRNA expression of 97% after incubation with TNF (5.75 nmol/l) for 24 hours, a decrease of 91% after 48 hours, and a decrease of 96% after 72 hours were measured. The reduction of protein expression was measured to be 42% after 24 hours, 28% after 48 hours, and 39% after 72 hours of incubation with TNF (5.75 nmol/l). The mRNA level of adiponectin receptor 1 (AdipoR1) was elevated about 72% after 48 hours of incubation and 67% after 72 hours of incubation, whereas the mRNA expression of adiponectin receptor 2 (AdipoR2) was not altered significantly. The visfatin mRNA level was found to be highly increased by 255% after 24 hours and 335% after 48 hours and 341% after 72 hours of incubation with TNF (5.75 nmol/l). Our results support the concept of visceral adipose tissue as an endocrine organ. We demonstrate that TNF has regulatory functions on adiponectin, AdipoR1 and on visfatin in human visceral adipose tissue. TNF levels are elevated in states of obesity and insulin resistance. Due to this fact TNF could be the reason that there is a decrease in the level of adiponectin, whereas there is an increase in the level of visfatin in states of obesity and insulin resistance.

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Correspondence

PD Dr. P. Algenstaedt

Department of Internal Medicine III

University Medical Center Hamburg-Eppendorf

Martinistraße 52

20246 Hamburg

Germany

Phone: +49/40/42803 47 55

Fax: +49/40/42803 68 20

Email: algensta@uke.uni-hamburg.de