Exp Clin Endocrinol Diabetes 2007; 115 - P01_001
DOI: 10.1055/s-2007-972257

Leptin directly controls ovarian functions in different species

AV Sirotkin 1, R Grossmann 2, HJ Schaeffer 3, J Rafay 1, A Benco 4, S Pavlova 4, A Bezakova 4, Z Kuklova 1, J Pivko 1, J Kotwica 5
  • 1Slovak Centre of Agricultural Studies, Research Institute of Animal Production, Nitra, Slovakia
  • 2Federal Agricultural Research Centre, Functional Genomics and Bioregulation, Neustadt am Rübenberge/Mariensee, Germany
  • 3University of Cologne, Institute of Pharmacology, Köln, Germany
  • 4Konstantin the Philosopher University, Nitra, Slovakia
  • 5Polish Academy of Sciences, Institute of Reproduction and Food Research, Olsztyn-Kortowo, Poland

Objectives: The aim of our in-vivo and in-vitro experiments was to examine the role of leptin in control of mammalian and avian ovarian functions.

Methods: Rabbits, pigs and chicken were subjected to food restriction, leptin treatment and their combination. Concentration of hormones in blood, as well as the release of hormones by ovarian cells, cultured with and without leptin, protein kinase blockers or after transfection with gene constructs for protein kinases and transcription factors, and the accumulation of substances within these cells was analysed by RIA, EIA, SDS PAGE-Western blotting, immunocytochemistry and TUNEL.

Results: It was observed that rabbits and pigs with high plasma leptin levels have reduced levels of ovarian steroid hormones and fertility. Leptin injections decreased levels of these hormones in rabbit plasma. Food restriction (50%) which decreased plasma leptin levels had no negative effect (chicken) or stimulated (rabbit) fertility. Exogenous leptin inhibited nuclear maturation of porcine oocytes, decreased proliferation (expression of PCNA, cyclin B1), regulated apoptosis (expression of bax, bcl-2, ASK-1, p53, TdT) and secretory activity (release of steroids, IGF-I, oxytocin, and prostaglandins) by cultured follicular tissue or granulosa cells isolated from rabbit, porcine and chicken ovaries. Blockers of protein kinases (PKA, MAPK, CDC2) and the transfection of cells with gene constructs induced overexpression of protein kinase ASK-1 and transcription factors STAT-1, p53 and NFkB were able to modify these leptin effects.

Conclusion: These observations suggest (1) that leptin could mediate the influence of nutrition on female mammalian and avian reproduction, (2) that leptin could be a potent and direct regulator of basic ovarian cell functions (proliferation, apoptosis, secretory and generative activity), and (3) that leptin can affect ovarian cells through PKA-, MAPK-, CDC2-, ASK-1-, STAT-1-, p53- and NFkB-dependent signalling pathways.