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Planta Med 1984; 50(3): 235-238
DOI: 10.1055/s-2007-969686
DOI: 10.1055/s-2007-969686
Research Articles
© Georg Thieme Verlag Stuttgart · New York
Radioimmunoassay for the Quantitative Determination of Quinine in Cultured Plant Tissues
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Publikationsverlauf
Publikationsdatum:
26. Februar 2007 (online)
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Abstract
The development of a radioimmunossay for the determination of picogram amounts of quinine is described. The antiserum, raised against a conjugate of quinine and bovine serum albumin, is highly specific to the Cinchona quinoline alkaloids having the 8S configuration and will not cross-react to any detectable extent with the 8R series. Neither will it cross-react with quininone or cinchonidinone, the common 9-keto precursors. Using this assay a number of clones of C. ledgeriana have been screened for their quinine content.