In Vitro Amoebicidal Testing of Natural Products; Part I. Methodology

Alison T. Keene; Ann Harris; J. David Phillipson; David C. Warhurst

doi:10.1055/s-2007-969152

Subscribe to RSS

Please copy the URL and add it into your RSS Feed Reader.

https://www.thieme-connect.de/rss/thieme/en/10.1055-s-00000058.xml

Share / Bookmark

Facebook X Linkedin Weibo

Download PDF

Planta Med 1986; 52(4): 278-285
DOI: 10.1055/s-2007-969152

Full Papers

In Vitro Amoebicidal Testing of Natural Products; Part I. Methodology

Alison T. Keene¹ , Ann Harris¹ , J. David Phillipson¹ , David C. Warhurst²

¹Department of Pharmacognosy, The School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, U. K.
²Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, University of London, Gower Street, London, WC1, U. K.

Further Information

Publication History

1985

Publication Date:
26 February 2007 (online)

Also available at

PDF Download Permissions and Reprints

Abstract

An in vitro test procedure utilising Entamoeba histolytica is described for the evaluation of crude extracts of plants and of isolated compounds. The test has been used to evaluate a number of standard antiamoebic drugs (emetine, 2, 3-dehydroemetine, metronidazole, 5-chloro-8-hydroxyquinoline), antimalarial drugs (amodiaquine, mepacrine, primaquine, chloroquine, quinine), Cinchona alkaloids (quinidine, quinidinone, cinchonamine, 10-methoxycinchonamine, 3-epiquinamine, aricine, crude extracts), quassinoids (bruceantin, bruceine C, quassin, Brucea crude extract) and the alkaloid canthin-6-one. The applicability of the test to screening plant extracts is discussed and the results are compared with in vitro cytoxicity tests to guinea-pig ear keratinocytes.