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DOI: 10.1055/s-2007-967114
In vitro Acetylcholinesterase Inhibitory Activity of the Essential Oil from Acorus calamus and its Main Constituents
Publikationsverlauf
Received: November 3, 2006
Accepted: January 7, 2007
Publikationsdatum:
07. Februar 2007 (online)
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Abstract
The in vitro acetylcholinesterase (AChE) inhibitory potential of the hydroalcoholic extract and of the essential oil from Acorus calamus (AC) rhizomes and that of its major constituents were evaluated based on the Ellman’s method. GC/MS analysis of the oil revealed that the major constituents were β-asarone (79.54 %) and α-asarone (8.47 %). The IC50 values were obtained for the hydroalcoholic extract, the essential oil, β-asarone and α-asarone and were 182.31 ± 16.78 μg/mL, 10.67 ± 0.81 μg/mL, 3.33 ± 0.02 μM and 46.38 ± 2.69 μM, respectively. Physostigmine was used as standard inhibitor with an IC50 value of 0.28 ± 0.015μM. The experimental observations revealed that the AC essential oil and its constituents have significant AChE inhibitory potential. β-Asarone, the major phytoconstituent present in the essential oil, showed the maximum inhibitory potential.
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References
- 1 Lahiri D K, Farlow M R, Greig N H, Sambamurti K. Current drug targets for Alzheimer’s disease treatment. Drug Dev Res. 2002; 56 267-81.
- 2 Lane R M, Kivipelto M, Greig N H. Acetylcholinesterase and its inhibition in Alzheimer disease. Clin Neuropharmacol. 2004; 27 141-9.
- 3 Vohora S B, Shah S A, Dandiya P C. Central nervous system studies on an ethanol extract of Acorus calamus rhizomes. J Ethnopharmacol. 1990; 28 53-62.
- 4 Oh M H, Houghton P J, Whang W K, Cho J H. Screening of Korean herbal medicines used to improve cognitive function for anti-cholinesterase activity. Phytomedicine. 2004; 11 544-8.
- 5 Howes M R, Houghton P J. Plants used in Chinese and Indian traditional medicine for improvement of memory and cognitive function. Pharmacol Biochem Behav. 2003; 75 513-27.
- 6 Dandiya P C, Cullumbine H, Sellers E A. Studies on Acorus calamus. IV, investigations on the mechanism of action of Acorus oil. J Pharmacol Exp Ther. 1959; 126 334-7.
- 7 Baxter R M, Dandiya P C, Kandel S I, Okany A, Walker G C. Separating of hypnotic potentiating principles from the essential oil of Acorus calumus L. of Indian origin by gas-liquid chromatography. Nature. 1960; 185 466-7.
- 8 Cho J S, Kim Y H, Kong J Y, Yang C H, Park C G. Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus . Life Sci. 2002; 71 591-9.
- 9 Manikandan S, Devi R S. Antioxidant property of α-asarone against noise-stress-induced changes in different regions of rat brain. Pharmacol Res. 2005; 52 467-74.
- 10 Oprean R, Oprean L, Tamas M, Sandulescu R, Roman L. Essential oils analysis. II. Mass spectra identification of terpene and phenylpropane derivatives. J Pharm Biomed Anal. 2001; 24 1163-8.
- 11 Raina V K, Srivastava S K, Syamasunder K V. Essential oil composition of Acorus calamus L. from the lower region of the Himalayas. Flavour Fragr J. 2003; 18 18-20.
- 12 Kumar V, Mal M, Saha B P, Mukherjee P K. Marker analysis of sweet flag (Acorus calamus L.) - a potent CNS active botanical used in Ayurveda. Philadelphia, PA; Drug Information Association 42nd Annual meeting 2006.
- 13 Manyam B V. Dementia in Ayurveda. J Altern Complement Med. 1999; 5 81-8.
- 14 Abel C. Chromosome-damaging effect of β-asarone on human lymphocytes. Planta Med. 1987; 53 251-3.
- 15 Hasheminejad G, Caldwell J. Genotoxicity of the alkenylbenzenes alpha- and beta-asarone, myristicin and elimicin as determined by the UDS assay in cultured rat hepatocytes. Food Chem Toxicol. 1994; 32 223-31.
- 16 The European Commission Scientific committee on Food. Opinion of the Scientific Committee on Food on the presence of β-asarone in flavourings and other food ingredients with flavouring properties. F/CS/FLAVOUR/9ADD1 Final January 8, 2002: 1-15.
- 17 Mukherjee P K. Quality control of herbal drugs - an approach to evaluation of botanicals. New Delhi; Business Horizons 2002: 197-205.
- 18 Ellman G L, Lourtney D K, Andres V, Gmelin G. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961; 7 88-95.
- 19 Ren Y, Houghton P J, Hider R C, Howes M JR. Novel diterpenoid acetylcholinesterase inhibitors from Salvia miltiorhiza . Planta Med. 2004; 70 201-4.
Dr. Pulok K. Mukherjee
School of Natural Product Studies
Department of Pharmaceutical Technology
Jadavpur University
Kolkata - 700032
India
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