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Horm Metab Res 1981; 13(4): 218-221
DOI: 10.1055/s-2007-1019225
© Georg Thieme Verlag, Stuttgart · New York

Thyroid Hormone Effects on β-Adrenergic Receptors in Isolated Fat Cells of Rats

Beate Pfeifle, R. Pfeifle, J.-D. Faulhaber, H. Ditschuneit
  • Zentrum für Innere Medizin der Universität Ulm, Abteilung Für Innere Medizin II, Ulm, Germany
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1979

1980

Publikationsdatum:
23. April 2008 (online)

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Summary

The effect of thyroid hormones (thyroxine and triiodothyronine) on catecholamine receptors in isolated rat fat cells was investigated.

Binding of (3H)isoproterenol and (3H)norepinephrine were increased by thyroid hormones. (3H)isoproterenol binding was more enhanced than (3H)norepinephrine binding.

Triiodothyronine had a more potent effect than thyroxine. (3H)isoproterenol was used to estimate the number or affinity of β-adrenergic receptors in rat fat cells treated with thyroid hormones. The binding sites for (3H)isoproterenol were the same in untreated and with triiodothyronine treated fat cells. The equilibrium dissociation constants (KD) for the interaction of receptors with (3H)isoproterenol were altered in thyroid hormone treated cells. There was a significant difference between the untreated and triiodothyronine treated fat cells in the affinity of β-adrenergic receptor binding sites for (3H)isoproterenol. Thyroid hormone could alter negative cooperative site-to-site interaction among the binding sites for (3H)isoproterenol.

Key-Words:

Thyroxine - Triiodothyronine - Catecholamine Receptors - Isolated Rat Fat Cells