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Horm Metab Res 1981; 13(3): 138-140
DOI: 10.1055/s-2007-1019200
ORIGINALS

© Georg Thieme Verlag, Stuttgart · New York

Effect of Ammonia on Glucose Oxidation by Isolated Rat Adipocytes

M. W. Stelling, F. A. Stephenson, A. D. Rogol
  • Departments of Pediatrics and Pharmacology University of Virginia Medical Center, Charlottesville, Virginia, U.S.A.
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Publication History

1979

1980

Publication Date:
14 March 2008 (online)

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Summary

The effect of ammonium and acetate salts on glucose metabolism in isolated rat adipocytes was studied. Ammonia in low concentration (1-10 mM) was found to inhibit insulin-stimulated CO2 production. Acetate had no effect on this system except at high concentrations (100 mM), at which it stimulated CO2 production. Ammonium and acetate salts had an anti-lipolytic effect at high concentration (100 mM) but no effect on lipolysis at lower concentrations.

The observation that trace amounts of salts can affect a biological assay system suggests that other biological assay system may also be altered by contaminating buffer salts from protein isolation steps.

Key-Words:

Adipocytes - Insulin - Glucose Oxidation - Lipolysis - Ammonium Salts