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Horm Metab Res 1981; 13(3): 135-138
DOI: 10.1055/s-2007-1019199
ORIGINALS

© Georg Thieme Verlag, Stuttgart · New York

Effect of Gut Peptides on Glucose-stimulated Insulin Release by Monolayer Cultures of Neonatal Rat Islet Cells

W. Y. Fujimoto
  • Division of Metabolism and Endocrinology, Department of Medicine RG-20, University of Washington, Seattle, WA, U.S.A.
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Publication History

1980

1980

Publication Date:
14 March 2008 (online)

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Summary

Gastric inhibitory polypeptide (GIP), pancreatic polypeptide (PP), glucagon, vasoactive intestinal polypeptide (VIP), bombesin, neurotensin, substance P, and cholecystokinin octapeptide (CCK-OP) were examined for their effects upon glucose-stimulated insulin secretion in denervated and isolated islet cells, namely, monolayer cultures of dispersed neonatal rat pancreatic islet ceils. Only glucagon (14 nM), GIP (10 and 20 nM), and CCK-OP (20 nM) enhanced glucose-stimulated insulin release during a 60-min incubation period. None of the others altered insulin secretion under the conditions employed, although reported to. influence insulin release in other systems.

Key-Words:

Islet Cell Monolayer Cultures - Insulin Secretion Glucagon - Gastric Inhibitory Polypeptide - Vasoactive Intestinal Polypeptide - Cholecystokinin - Bombesin - Neurotensin - Pancreatic Polypeptide - Substance P.