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Horm Metab Res 1983; 15(11): 557-561
DOI: 10.1055/s-2007-1018787
© Georg Thieme Verlag, Stuttgart · New York

Hydrocortisone and 1,25(OH)2 D3: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

L. M. Brewer, R. A. Corradino
  • Department of Physiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York, U.S.A.
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Publication History

1982

1982

Publication Date:
14 March 2008 (online)

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Summary

The present studies were conducted to determine if hydrocortisone (HC) and/or 1,25-dihydroxycholecalciferol (1,25(OH)2 D3) alter proliferative responses of the cultured duodenum, and if such alterations could be related to the known augmentation of 1,25(OH)2 D3-induction of a specific, intestinal calcium-binding protein (CaBP) by glucocorticoids. HC stimulated proliferation in the duodenal epithelium, as indicated by increased DNA synthesis (3H-thymidine uptake), increased ceil number/villus, and increased mitotic index after colchicine treatment. Goblet cell numbers were not significantly increased with any hormone treatment. The presence of 1,25 (OH)2, D3, alone did not affect proliferative responses. CaBP concentration as a function of tissue weight was 50% greater in HC stimulated intestine, indicating that the proliferogenic action of HC on the duodenum alone could not account for the glucocorticoid-1,25(OH)2, D3 interaction in CaBP synthesis.

Key-Words:

Hydrocortisone - 1,25(OH) 2 D 3 - Embryonic Chick Duodenum - Proliferation in Organ Culture

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