Insulin Receptor of Erythrocytes and Glucose Metabolism in Uremic Children

Summary

This study represents the results of oral glucose tolerance test (O-GTT) of 28 children with chronic renal failure, including 12 undialyzed and 16 dialyzed cases, compared to their own insulin receptors of erythrocytes. 7 of the 12 undialyzed patients (58.3%) represented abnormal O-GTT, while in dialyzed patients 3 of 16 cases (18.8%) had abnormal O-GTT. The maximum binding of ¹²⁵I-insulin to erythrocytes, 6.34 ± 1.93% in dialyzed patients, was significantly lower (p < 0.05) than 7.73 ± 1.03% in controls, and 4.87 ± 1.01% in undialyzed patients was significantly lower than both dialyzed (p < 0.05) and controls (p < 0.001). In undialyzed patients, 350 ± 221 binding sites/cell of insulin receptor was significantly fewer than 544 ± 207 binding sites/cell in controls (p < 0.05), but no significance was detected when compared to the 562 ± 331 binding sites/cell in dialyzed patients, because of the large variation in dialyzed patients. The average affinity of empty site (Ke × 10⁷/M) that 4.18 ± 2.73 in undialyzed patients, 3.40 ± 2.32 in dialyzed patients and 3.37 ± 1.05 in controls, had no significance between any two of the three. There were no significant correlations between receptor concentration and the sum of blood sugar (ΣBS) or the sum of insulin (ΣIRI), and no correlation between receptor concentration and BUN or creatinine. Furthermore, no significant relationships could be found when comparing BUN or creatinine with O-GTT. However, if the patients were divided into groups as normal and abnormal of O-GTT, significantly higher values of ΣBS (p < 0.001) and lower concentrations of insulin receptor (p < 0.05) were found in the abnormal group. No difference was found in ΣIRI and Ke. In addition, a significantly lower value of serum calcium concentration was found in the abnormal group of O-GTT.

Key-Words:

Insulin Receptor-Erythrocyte-Uremia - Hemodialysis-Glucose Metabolism