Cyclic AMP Release from Perfused Dog Thyroid Lobes as a Sensitive Indicator of TSH Activation of the Secretion Process. Evidence for Two Types of Thyroid Secretion Latency

Summary

It has been demonstrated in various types of thyroid tissue preparations that cyclic AMP (cAMP) released into the medium reflects the amount of cAMP in the cells. In the present study employing perfused dog thyroid lobes the dynamics of cAMP release were compared to those of thyroxine (T₄) and triiodothyronine (T₃) release. The experiments gave evidence that

a: even the lowest concentrations of TSH which stimulate hormone release (in this study 1 μU/ml) also activate the cAMP system;

b: the very high levels of cAMP obtained by stimulation with high concentrations of TSH (in this study 10,000 μU/ml) are not accompanied by corresponding high increases in hormone release. On the contrary the T₄ and T₃ release is lower than during stimulation with more moderate concentrations of TSH (100 μU/ml). Hence studies employing high concentrations of TSH and measurements of cAMP as indicator of activity of secretory processes should be interpreted very cautiously;

c: the prolonged lag in thyroid hormone secretion observed after stimulation with low concentrations of TSH is accompanied by a corresponding lag in activation of the cAMP system. This pattern suggest that the duration of late secretory processes such as thyroglobulin pinocytosis and hydrolysis is independent of the degree of stimulation and not involved in the variations in secretion latency.

Key-Words:

cAMP - Thyroxine - 3,3′,5-Triiodothyronine - Desensitization - Secretion Latency - TSH