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Horm Metab Res 1986; 18(2): 82-87
DOI: 10.1055/s-2007-1012237
ORIGINALS
Basic
© Georg Thieme Verlag, Stuttgart · New York

Extracts of Protozoa Contain Materials That React Specifically in the Immunoassay for Guinea Pig Insulin

Flora de Pablo, M. A. Lesniak, E. R. Hernandez, D. LeRoith, J. Shiloach1 , J. Roth
  • Diabetes Branch, National Institute of Arthritis, Diabetes and Digestive, and Kidney Diseases, Bethesda, Maryland, U.S.A.
  • 1Pilot Plant, NIADDK, National Institutes of Health, Bethesda, Maryland, U.S.A.
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Publication History

1984

1984

Publication Date:
14 March 2008 (online)

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Summary

Extracts of protozoa contain materials that resemble guinea pig insulin, which is noted for its unusual structure and properties. The protozoan derived materials react in the radioimmunoassay for guinea pig insulin; some but not all of these immunoactive materials migrate on gel filtration in the position of authentic guinea pig insulin. Experiments were done to exclude artifacts in the assay as well as inadvertent contamination by guinea pig insulin. By immunological methods, we segregated the guinea pig type immunoactivity from that which has rat/pork type immunoactivity. These findings extend our studies of extracts of guinea pig tissues which also have these two types of insulin immunoactivities.

Key-Words

Rat/Pork Insulin - Guinea Pig Insulin - Radioimmunoassay - Tetrahymena - Immunodepletion