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Horm Metab Res 1989; 21(8): 431-433
DOI: 10.1055/s-2007-1009254
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© Georg Thieme Verlag, Stuttgart · New York

Column Perifusion System for Steroidogenesis in Isolated Rat Adrenal Cells

M. Matsuki1 , M. Ikeda2 , S. Watanabe3 , S. Nishida1 , M. Horino1 , T. Saito3
  • 1Department of Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan
  • 2Department of Biochemistry, Kawasaki Medical School, Kurashiki, Okayama, Japan
  • 3Department of Pharmacology, Kawasaki Medical School, Kurashiki, Okayama, Japan
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Publication History

1988

1988

Publication Date:
14 March 2008 (online)

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Summary

A perifusion system using a plastic column into which isolated rat adrenal cells had been installed was attempted. After ACTH or cAMP was administered to the column, the corticosterone concentration in the eluate was determined. ACTH in 10-13 and 10-12 M did not promote corticosterone production, whereas 10-11 and 10-10 M showed a dose dependent production of corticosterone. By iterative infusion of 10-11 or 10-9 M of ACTH, very clear responses to restimulation of ACTH were noted. Following the administrations of 10-3 or 10-2 M of dibutyryl adenosine 3′,5′-cyclic monophosphate (dbcAMP), the production of corticosterone increased dose-dependently. These results suggest that this perifusion system is effective for examining the effects of ACTH or cAMP on Steroidogenesis of cells.

Key words

Column Perifusion - Rat - Adrenal Cells

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