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DOI: 10.1055/s-2007-1004855
© Georg Thieme Verlag, Stuttgart · New York
Presence of High Affinity Receptor for Interleukin-1 (IL-1) on Cultured Porcine Thyroid Cells
Publikationsverlauf
1988
1989
Publikationsdatum:
14. März 2008 (online)
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Summary
Using 125I-interleukin-1β (125I-IL-1β) as a ligand, a specific receptor of high affinity dissociation constant (1.1 ± 0.2 × 10-10 M) with binding sites (350 ± 40/cell) for interleukin-1β (IL-1β) has been demonstrated on cultured porcine thyroid cells. IL-1α Almost equally cross-reacted with the receptor (Kd = 1.2 ± 0.3 × 10-10 M and 350 ± 50 binding sites/cell). TSH, IL-2 and other peptide hormones did not inhibit the binding of 125I-IL-1β to thyroid cells.
Crosslinking study revealed a major band (∼ 95 kD) with a corrected molecular mass of ∼ 78 kD. Moreover, both IL-1β and IL-1α stimulated prostaglandin E2 production of cultured porcine thyroid cells, although the potency of IL-1α was slightly greater than that of IL-1β. These results suggest that IL-1 may be involved in the regulation of thyroid cell function.
Key words
Interleukin-1 - Receptor - Thyroid cells - Prostaglandin E2