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Horm Metab Res 1990; 22(2): 61-64
DOI: 10.1055/s-2007-1004852
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© Georg Thieme Verlag, Stuttgart · New York

Potentiation of Forskolin-Induced Increase of cAMP by Diamide and N-Ethylmaleimide in Rat Pancreatic Islets

M. I. Anazodo, A. B. Müller, H. Safayhi, H. P. T. Ammon
  • Pharmazeutisches Institut, Abteilung Pharmakologie, Universität Tübingen, Tübingen, Germany
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Publication History

1989

1989

Publication Date:
14 March 2008 (online)

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Summary

In isolated rat pancreatic islets, the effect of diamide and N-ethylmaleimide (NEM) on forskolin- as well as on glucagon-induced elevation of cAMP was studied. Forskolin and glucagon increased cAMP levels in batch-incubated islets. Diamide and NEM further augmented forskolin-induced increase of cAMP levels, whereas glucagon-stimulated elevation of cAMP was not affected. From our data it is likely that under the conditions of the present study, the thiols related to the Ns-protein and the catalytic unit are insensitive to oxidation and alkylation. The potentiation of forskolin-induced cAMP production in intact islet cells by diamide and NEM may be due to inactivation of the thiols related to the Ni-protein.

Key words

Pancreatic Islets - Ni-Protein - Forskolin - Thiolreagents