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Horm Metab Res 1991; 23(8): 362-364
DOI: 10.1055/s-2007-1003701
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© Georg Thieme Verlag, Stuttgart · New York

Increased Insulin Responsiveness in Isolated Rat Hepatocytes Incubated with Free Fatty Acid-Poor Albumin1

J. F. Caro, J. Hodges, M. K. Sinha
  • Department of Medicine, Section of Endocrinology and Metabolism, East Carolina University School of Medicine, Greenville, North Carolina, U. S. A.
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Publication History

1989

1991

Publication Date:
14 March 2008 (online)

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Summary

Freshly isolated rat hepatocytes incubated in a medium containing bovine serum albumin partially purified by charcoal treatment at pH 3 are three times more responsive to insulin with regard to α-aminoisobutyric acid uptake than those incubated in medium containing regular bovine serum albumin. This finding should facilitate future work in isolated hepatocytes, a cell system that for the most part has been considered relatively unresponsive to insulin.

Key words

Insulin - Hepatocytes - Insulin Action - Fatty Acid-free Albumin - Bovine Serum Albumin