Horm Metab Res 1992; 24(7): 326-328
DOI: 10.1055/s-2007-1003324
Originals Basic

© Georg Thieme Verlag, Stuttgart · New York

Electrophysiological Changes of Sertoli Cells Produced by the Acute Administration of Amino Acid and FSH

G. F. Wassermann1 , L. Monti Bloch2 , M. L. Grillo1 , F. R. M. B. Silva1 , E. S. Loss1 , L. L. McConnell1
  • 1Center of Experimental Endocrinology, Institute of Biosciences, UFRGS, Porto Alegre, RS, Brazil
  • 2Department of Physiology, School of Medicine, University of the Republic, Montevideo, Uruguay
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Publikationsverlauf

1990

1991

Publikationsdatum:
14. März 2008 (online)

Summary

Electrophysiological studies were carried out using seminiferous tubules of “Sertoli cell enriched testes” of rats irradiated in utero. Sertoli cells were inserted with glass microelectrodes in a superfision chamber continuously perfused with KRb buffer. The topical application of FSH (4.0 mU/ml) produced a biphasic effect characterized by a rapid hyperpolarization (< 3 s) followed by depolarization. The depolarizing effect of FSH was prolonged and potentiated in the presence of 5 mmol/l α-methylaminoisobutyric acid in the bath medium of the superfusion chamber. Verapamil, at a dose (250 μmol/1) that nullified the stimulatory action of FSH in the amino acid transport, suppressed the depolarizing effect of FSH. It was concluded that in immature rat testes FSH produces electrophysiological changes that mediate the stimulatory action of the amino acid transport.