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Planta Med 1988; 54(5): 426-430
DOI: 10.1055/s-2006-962489
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© Georg Thieme Verlag Stuttgart · New York

TLC and HPLC Analysis of Echinacea pallida and E. angustifolia Roots1

R. Bauer, I. A. Khan, H. Wagner
  • Institut für Pharmazeutische Biologie, Universität München, Karlstraße 29, D-8000 München 2, Federal Republic of Germany.
1 Part 4 in the series “Echinacea”. For part 3 see Bauer, R., Remiger, P., Wagner, H. (1988) Dtsch. Apoth. Ztg. 128, 174-180.
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Publication History

1988

Publication Date:
24 January 2007 (online)

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Abstract

The roots of Echinacea pallida are characterized by TLC and HPLC fingerprints of the polyacetylenes, polyenes, and the caffeic acid derivatives. The fingerprints can also be used for the differentiation between E. pallida and E. angustifolia root extracts. The main constituents of E. pallida roots are tetradeca-8Z-ene-11,13-diyn-2-one (2), pentadeca-8Z-ene-11,13-diyn-2-one (3), pentadeca-8Z,13Z-dien-11-yn-2-one (4), pentadeca-8Z,11Z,13E-trien-2-one (5), pentadeca-8Z,11E,13Z-trien-2-one (6), pentadeca-8Z,11Z-dien-2-one (7), and echinacoside (13). E. angustifolia is usually void of these polyacetylenes and polyenes and is characterized by isobutylamides, echinacoside, and cynarine. The polyacetylenes of E. pallida are very susceptible to autoxidation to the 8-hydroxy-9-ene derivatives. Thus, the chemical composition of the roots depends on the storage conditions.