Embryoid Formation by High Temperature Treatment from Multiple Shoots of Panax ginseng1

Isao Asaka; Ichio li; Takafumi Yoshikawa; Masao Hirotani; Tsutomu Furuya

doi:10.1055/s-2006-959697

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Planta Med 1993; 59(4): 345-346
DOI: 10.1055/s-2006-959697

Papers

Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Embryoid Formation by High Temperature Treatment from Multiple Shoots of Panax ginseng ¹

Isao Asaka² , Ichio li² , Takafumi Yoshikawa³ , Masao Hirotani³ , Tsutomu Furuya³

²Life Science Center, Iwaki Glass Co. Ltd., Gyoda, Funabashi-shi, Chiba 273, Japan
³School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 108, Japan

1 Part 83 in the series “Studies on Plant Tissue Culture”. For Part 82 see Ref.

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Publication History

1992

1992

Publication Date:
04 January 2007 (online)

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Abstract

We developed a new technology to induce embryoids by a moderate high temperature treatment from multiple shoots of Panax ginseng (Araliaceae). The number of formed embryoids was 10 times higher than that of untreated tissue. Normal plantlets were regenerated from the embryoids by transplanting them on a hormone-free medium. They contained ginsenosides Rb₁, Rg₁ and the other saponins as well as those of natural ginseng.

Key words

Panax ginseng - Araliaceae - plant tissue culture - embryoid formation - temperature treatment - mass propagation