Subscribe to RSS
DOI: 10.1055/s-2006-959444
© Georg Thieme Verlag Stuttgart · New York
Effect of a Peptide from Panax ginseng on the Proliferation of Baby Hamster Kidney-21 Cells
Publication History
1993
1993
Publication Date:
04 January 2007 (online)
![](https://www.thieme-connect.de/media/plantamedica/199402/lookinside/thumbnails/10.1055-s-2006-959444-1.jpg)
Abstract
An alkaline fraction separated by ion exchange chromatographies from the water extract of Panax ginseng root stimulated the proliferation of baby hamster kidney-21 cells. Separation of the alkaline fraction by MCI-gel CHP 20P column chromatography followed by dialysis provided an active material. By a reversed-phase HPLC the active material was separated into six fractions, and an active colorless Compound 1 was obtained from fraction 2 in a pure State. Compound 1 was composed of the following amino acids; Gly, Arg, Glu, Val in a ratio of 1:1:1:1, and caused 20% enhancement of proliferation of BHK-21 cells at a concentration of 3.40 µM. On the basis of physical and spectral data the structure of Compound 1 was established as a tetrapeptide, Gly-Arg-γ-Glu-Val-NH2.
Key words
Panax ginseng - Araliaceae - baby hamster kidney-21 cells - proliferation - tetrapeptide