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Planta Med 1994; 60(2): 171-174
DOI: 10.1055/s-2006-959444
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© Georg Thieme Verlag Stuttgart · New York

Effect of a Peptide from Panax ginseng on the Proliferation of Baby Hamster Kidney-21 Cells

Akira Yagi1 , Kazuko Akita, Tadashi Ueda2 , Nobuyuki Okamura1 , Hiroshi Itoh3
  • 1Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima, 729-02, Japan
  • 2Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812, Japan
  • 3Maruzen Pharmaceutical Co. Ltd., Onomichi, Hiroshima, 722, Japan
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Publication History

1993

1993

Publication Date:
04 January 2007 (online)

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Abstract

An alkaline fraction separated by ion exchange chromatographies from the water extract of Panax ginseng root stimulated the proliferation of baby hamster kidney-21 cells. Separation of the alkaline fraction by MCI-gel CHP 20P column chromatography followed by dialysis provided an active material. By a reversed-phase HPLC the active material was separated into six fractions, and an active colorless Compound 1 was obtained from fraction 2 in a pure State. Compound 1 was composed of the following amino acids; Gly, Arg, Glu, Val in a ratio of 1:1:1:1, and caused 20% enhancement of proliferation of BHK-21 cells at a concentration of 3.40 µM. On the basis of physical and spectral data the structure of Compound 1 was established as a tetrapeptide, Gly-Arg-γ-Glu-Val-NH2.

Key words

Panax ginseng - Araliaceae - baby hamster kidney-21 cells - proliferation - tetrapeptide