Planta Med 1994; 60(1): 41-44
DOI: 10.1055/s-2006-959405
Paper

© Georg Thieme Verlag Stuttgart · New York

Antifungal Tests in Phytochemical Investigations: Comparison of Bioautographic Methods using Phytopathogenic and Human Pathogenic Fungi

L. Rahalison1 , M. Hamburger1 , M. Monod2 , E. Frenk2 , K. Hostettmann1
  • 1Institut de Pharmacognosie et Phytochimie, Université de Lausanne, B.E.P., CH-1015 Lausanne, Switzerland
  • 2Laboratoire de Mycologie, Service de Dermatologie, Centre Hospitalier Universitaire Vaudois, C.H.U.V., CH-1011 Lausanne, Switzerland
Further Information

Publication History

1993

1993

Publication Date:
04 January 2007 (online)

Abstract

The detection limits in two bioautographic assays have been determined for a series of antifungal Compounds, including clinically used anti-mycotics, fungicidal agrochemicals, and various classes of secondary plant metabolites. Target organisms were the filamentous fungus Cladosporium cucumerinum and the yeast Candida albicans. For clinical agents and agrochemicals, the detection limits in the two assays re-flected to a certain extent their known spectrum of activity. Most of the plant-derived Compounds tested showed a positive response in both assays, but with detection limits varying by a factor up to tenfold. For Screening purposes, it is thus advisable to use both tests, as some activities would otherwise go undetected. The MIC values of these substances were determined in Order to verify a possible correlation with the detection limit in the bioautographic assays.