Download PDF
Planta Med 1997; 63(1): 75-79
DOI: 10.1055/s-2006-957609
Papers
Phytochemical Analysis
© Georg Thieme Verlag Stuttgart · New York

Method for the Simultaneous Determination of Aconitum Alkaloids and their Hydrolysis Products by Gas Chromatography-Mass Spectrometry in Human Serum

Kitae Ito, Yoshiharu Ohyama, Yuko Konishi, Satoshi Tanaka, Michinao Mizugaki
  • Department of Pharmaceutical Sciences, Tohoku University Hospital 1-1 Seiryo-machi, Aoba-ku, Sendai 980-77, Japan
Further Information

Publication History

1996

1996

Publication Date:
04 January 2007 (online)

Also available at
  PDF Download  Permissions and Reprints

Abstract

This paper describes a newly developed method using gas chromatography-selected ion monitoring (GC-SIM) for the simultaneous determination of diester-diterpene type Aconitum alkaloids and their hydrolysis products in human serum. When the alkaloids were converted into their TMS derivatives, they produced a well defined peak on selected ion recording (SIR). Good linear response over the range of 100 pg to 7.5 ng was demonstrated for each alkaloid. When the alkaloids were spiked into human serum, good quantitative recovery was observed.

Key words

Ranunculaceae - Aconitum alkaloids - GC-SIM - determination of Aconitum alkaloids - human serum