Isolation of Lipolytic Substances Caffeine and 1,7-Dimethylxanthine from the Stem and Rhizome of Sinomenium actum

Ming Jiang; Kenji Kameda; Li-Kun Han; Yoshiyuki Kimura; Hiromichi Okuda

doi:10.1055/s-2006-957456

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Planta Med 1998; 64(4): 375-377
DOI: 10.1055/s-2006-957456

Letters

Isolation of Lipolytic Substances Caffeine and 1,7-Dimethylxanthine from the Stem and Rhizome of Sinomenium actum

Ming Jiang¹ , Kenji Kameda² , Li-Kun Han¹ , Yoshiyuki Kimura¹ , Hiromichi Okuda¹

¹Second Department of Medical Biochemistry, Ehime University, Shigenobu-cho, Onsen-gun, Ehime, Japan
²Central Research Laboratory, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime, Japan

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Publication History

1997

1997

Publication Date:
04 January 2007 (online)

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Abstract

We attempted to isolate lipolytic substances from the stem and rhizome of Sinomenium actum Rehder et Wilson by using high-performance liquid chromatography (HPLC). S-I and S-II were isolated from the fractions showing lipolytic activity. S-I and S-II were identified as caffeine and 1,7-dimethylxanthine, respectively, by direct comparison with authentic samples. Caffeine (S-I) dose-dependently stimulated lipolytic activity in isolated fat cells of rats, at concentrations of 500 to 1000 µM. 1,7-Dimethylxanthine (S-II) also stimulated lipolytic activity at concentrations of 500 to 1000 µM. Furthermore, we found that caffeine and 1,7-dimethylxanthine enhanced catecholamine-induced lipolysis at lower concentrations of 0.1 to 1 µM.