Planta Med 2006; 72(13): 1223-1226
DOI: 10.1055/s-2006-951695
Original Paper
Biochemistry and Molecular Biology
© Georg Thieme Verlag KG Stuttgart · New York

Molecular Authentication of the Traditional Tibetan Medicinal Plant Swertia mussotii

Chun-Ying Xue1 , De-Zhu Li1 , Jin-Mei Lu1 , Jun-Bo Yang1 , Jian-Quan Liu2
  • 1Laboratory of Plant Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan P. R. China
  • 2Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, QingHai, P. R. China
Weitere Informationen

Publikationsverlauf

Received: December 5, 2005

Accepted: August 25, 2006

Publikationsdatum:
18. Oktober 2006 (online)

Abstract

Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region.

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Dr. Chun-Ying Xue

Laboratory of Plant Biodiversity and Biogeography

Kunming Institute of Botany

Chinese Academy of Sciences

Kunming 650204

Yunnan

People’s Republic of China

Telefon: +86-871-522-3508

Fax: +86-871-521-7791

eMail: chyxue@mail.kib.ac.cn