PDF herunterladen
Exp Clin Endocrinol Diabetes 2006; 114(10): 563-568
DOI: 10.1055/s-2006-948307
Article

© J. A. Barth Verlag in Georg Thieme Verlag KG · Stuttgart · New York

Protective Effect of Gui Qi Mixture on the Progression of Diabetic Nephropathy in Rats

Y.-W. Zhang 1 , D. Xie 1 , Y.-X. Chen 1 , H.-Y. Zhang 1 , Z.-X. Xia 1
  • 1Department of Chinese with Western Medicine, Zhongnan Hospital, Wuhan University, Wuhan, China
Weitere Informationen

Publikationsverlauf

Received: January 4, 2006 First decision: May 26, 2006

Accepted: June 21, 2006

Publikationsdatum:
19. Dezember 2006 (online)

Auch verfügbar auf
    Lizenzen und Reprints

Abstract

Huang Qi (root of Astragalus membranaceus) and Dang Gui (Angelica sinensis), two of the most widely used herbs in traditional Chinese medicine, have been proven to be effective in the treatment of diabetes mellitus (DM) although the underlying molecular mechanisms are not fully elucidated. This study was designed to investigate the protective effect of Dang Gui and Huang Qi mixture (GQM) on the development of diabetic nephropathy in rats with streptozotocin (STZ)-induced DM and the possible underlying molecular mechanism. The diabetic animal model was made by a single intraperitoneal injection of STZ and then treated with GQM or benazepril. Blood glucose, triglyceride (TG), cholesterol (CHO), high density lipoprotein (HDL), serum creatinine (Scr), creatinine clearance rate (Ccr), blood urea nitrogen (BUN), urine β2-microglobin (β2-MG), kidney/body weight (K/B) ratio, glomerular area (GA), renal transforming growth factor-β1 (TGF-β1) mRNA expression and blood and renal angiotensin II (AngII) expression were determined 8 weeks after the treatment. The blood glucose, CHO and TG levels, BUN, SCr, Ccr. K/B ratio, GA, the excretion of β2-MG, renal TGF-β1 mRNA expression and blood and renal AngII expression were significantly increased while the HDL level was decreased 8 week after STZ injection. The changes in blood glucose, TG, CHO and HDL were reversed by GQM, not by benazepril, whereas the changes in other variables were reversed by both GQM and benazepril. Our results suggest that GQM alleviates the disorder in blood glucose and lipids, protects against the progression of renal nephropathy in diabetic rats, probably by inhibiting the expression of AngII and TGF-β1 mRNA.

Key words

Diabetic nephropathy - Astragalus membranaceus - Angelica sinensis - traditional Chinese medicine

References

  • 1 Border WA, Noble NA. Transforming growth factor beta in tissue fibrosis.  N Engl J Med. 1994;  331 1286-1292
    CrossrefPubMedGoogle Scholar
  • 2 Branton MH, Kopp JB. TGF-beta and fibrosis.  Microbes Infect. 1999;  1 1349-1365
    CrossrefPubMedGoogle Scholar
  • 3 Bruno G, Merletti F, Biggeri A, Bargero G, Ferrero S, Pagano G, Cavallo-Perin P. Casale Monferrato Study: Progression to overt nephropathy in type 2 diabetes: the Casale Monferrato Study.  Diabetes Care. 2003;  26 2150-2155
    PubMedGoogle Scholar
  • 4 Chen D, Gong D, Zhai Y. Clinical and experimental studies in treating diabetes mellitus by acupuncture.  J Tradit Chin Med. 1994;  14 163-166
    PubMedGoogle Scholar
  • 5 Chen KK. Pharmacology of Oriental Plants. First Edition. Pergamon Press London 1965
    Google Scholar
  • 6 Dan X, Zhang YW. Effect of Guiqi Mixture on transforming growth factor-beta1 in renal tissue of diabetic rats.  Med J Wuhan Univ. 2004;  25 655-659 , (Chinese)
    PubMedGoogle Scholar
  • 7 Du Y, Yao A, Guo DF, Inagami T, Wang DH. Differential regulation of angiotensin II receptor subtypes in rat kidney by low dietary sodium.  Hypertension. 1995;  25 872-877
    PubMedGoogle Scholar
  • 8 Fan JM, Liu LS, Li Z, Su BH, Guan J. A Meta-analysis of radix astragali for primary nephritic syndrome in adults.  Tradit Chin Drug Res Clin Pharmacol. 2003;  14 62-66 , (Chinese)
    PubMedGoogle Scholar
  • 9 Han H, Fan YY, Zhang KJ, Xu YC. Effects of angelica solution injection on hemorrheology and coagulationin type 2 diabetes mellitus patients.  Med J Wuhan Univ. 2003;  24 65-67 , (Chinese)
    PubMedGoogle Scholar
  • 10 He SQ, Fan ZY, Yu LF. Overview and analysis of Chinese medicine in treating diabetic peripheral neuropathy.  Shanghai J Tradit Chin Med. 2004;  38 60-62 , (Chinese)
    PubMedGoogle Scholar
  • 11 Hong H. Progress of experimental studies on hypoglycemic mechanisms by TCM.  J Anhui Tradit Chin Med Col. 2001;  20 59-63 , (Chinese)
    PubMedGoogle Scholar
  • 12 Jiang LJ, Li L. Research progress of the therapeutic mechanisms of diabetic complications with active ingredients from TCM.  J Anhui Tradit Chin Med Col. 2001;  20 63-65 , (Chinese)
    PubMedGoogle Scholar
  • 13 Li B, Zhou W, Li P. Protective effects of nifedipine and allopurinol on high energy shock wave induced acute changes of renal function.  J Urol. 1995;  153 596-598
    CrossrefPubMedGoogle Scholar
  • 14 Li JH, Huang XR, Zhu HJ, Johnson R, Lan HY. Role of TGF-beta signaling in extracellular matrix productionunder high glucose conditions.  Kidney Int. 2003;  63 2010-2019
    CrossrefPubMedGoogle Scholar
  • 15 Li QM. The mechanisms of TCM for the treatment of diabetes.  Chin J Exp Tradit Med Form. 2000;  6 56-58 , (Chinese)
    PubMedGoogle Scholar
  • 16 Liu TH, Lu RH. The mechanisms of TCM for the prevention of diabetes and its complications.  J Beijing Univ Tradit Chin Med. 2000;  23 69-71 , (Chinese)
    PubMedGoogle Scholar
  • 17 Liu ZQ, Li QZ, Qin GJ. Effect of Astragalus injection on platelet function and plasma endothelin in patients with early stage diabetic nephropathy.  Chin J Integr Tradit Western Med. 2001;  21 274-276 , (Chinese)
    PubMedGoogle Scholar
  • 18 Maschio G, Alberti D, Janin G, Locatelli F, Mann JF, Motolese M, Ponticelli C, Ritz E, Zucchelli P. Effect of the angiotensin-converting-enzyme inhibitor benazepril on the progression of chronic renal insufficiency. The angiotensin-converting-enzyme inhibition in progressive renal insufficiency Study Group.  N Engl J Med. 1996;  334 939-945
    CrossrefPubMedGoogle Scholar
  • 19 Reddy S, Wu D, Elliott RB. Low dose streptozotocin causes diabetes in severe combined immunodeficient (SCID) mice without immune cell infiltration in the pancreatic islets.  Autoimmunity. 1995;  20 83-92
    PubMedGoogle Scholar
  • 20 Rees DA, Alcolado JC. Animal models of diabetes mellitus.  Diabet Med. 2005;  22 359-370
    CrossrefPubMedGoogle Scholar
  • 21 Shi P, Huang LM, Liang H, Pang LX, Zhao H. A study of effects of extracts from radix astragli on IgA nephrosis in mouse.  China Pharmaceutical. 2004;  13 36-38 , (Chinese)
    PubMedGoogle Scholar
  • 22 Singh R, Singh AK, Leehey DJ. A novel mechanism for angiotensin II formation in streptozotocin-diabetic rat glomeruli.  Am J Physiol Renal Physiol. 2005;  288 F1183-1190
    CrossrefPubMedGoogle Scholar
  • 23 Suzuki Y, Ruiz-Ortega M, Egido J. Angiotensin II: a double-edged sword in inflammation.  J Nephrol. 2000;  13 ((Suppl 3)) S101-110
    PubMedGoogle Scholar
  • 24 Tanimoto K, Tamura K, Sugiyama F, Murakami K, Fukamizu A. Isolation of the mouse Ren-1C gene and characterization of renin gene expression in both ES-D3 cells and their parental mouse strain.  J Reprod Dev. 1993;  39 19-24
    PubMedGoogle Scholar
  • 25 Vinikor F. Is diabetes a public health disorder?.  Diabetes Care. 1994;  17 ((Suppl 1)) 22-27
    PubMedGoogle Scholar
  • 26 Wang L, Li SL, Wang T. Progress on the study of polysaccharides and glucosides of hypoglycemic plants.  J Chin Med Materials. 2000;  23 575-577 , (Chinese)
    PubMedGoogle Scholar
  • 27 Xie KM, Mao Y, Xie P, Gu YP. Inhibitory effects of Angelicae sinensis and sodium ferulate on acute inflammatory liver injury and expression of ICAM-1 and E-selectin in mice.  Chin J Pathophysiol. 2004;  20 2330-2335 , (Chinese)
    PubMedGoogle Scholar
  • 28 Zhang HF, Li SM. Research progress of natural hypoglycemic agents.  Chin Tradit Herb Drugs. 2000;  31 184-186 , (Chinese)
    PubMedGoogle Scholar
  • 29 Zhang SL, To C, Chen X, Filep JG, Tang SS, Ingelfinger JR, Chan JS. Essential role(s) of the intrarenal renin-angiotensin system in transforming growth factor-beta1 gene expression and induction of hypertrophy of rat kidney proximal tubular cells in high glucose.  J Am Soc Nephrol. 2002;  13 302-312
    PubMedGoogle Scholar
  • 30 Zhang YW, Xie D. Protective effect of Danggui Buxue decoction on the kidney of diabetic rats.  Chin Tradit Patent Med. 2005;  27 75-78 , (Chinese)
    PubMedGoogle Scholar
  • 31 Zhang YW, Xie D. Protective effect of Guiqi mixture on renal diseases of early stage in diabetic rats.  Chin J Integr Tradit Western Med (Digest). 2005;  13 22-25 , (Chinese)
    PubMedGoogle Scholar
  • 32 Zhao L, Yang LX. Clinical analysis on effect of Milkvetch root injection in treating diabetic nephropathy.  Chin J IntegrTradit Western Med. 2005;  12 8-9 , (Chinese)
    PubMedGoogle Scholar

Correspondence

Prof. Ying-Wen Zhang

Department of Chinese with Western Medicine

Zhongnan Hospitial

Wuhan University

430071 Wuhan

China

Telefon: +86/2763/25 76 86

Fax: +86/2727/87 33 07 95

eMail: hhao3838@yahoo.com.cn