Horm Metab Res 2006; 38(1): 28-33
DOI: 10.1055/s-2006-924973
Original Clinical
© Georg Thieme Verlag KG Stuttgart · New York

Adipose Tissue and Circulating Endothelial Cell Specific Molecule-1 in Human Obesity

J.  Janke1 , S.  Engeli1 , K.  Gorzelniak1 , M.  Feldpausch1 , U.  Heintze1 , J.  Böhnke1 , M.  Wellner1 , F.  Herse1 , P.  Lassalle2 , F.  C.  Luft1 , A.  M.  Sharma3
  • 1Medical Faculty of the Charité, Franz Volhard Clinic, HELIOS Klinikum-Berlin, Germany
  • 2INSERM U416, Institut Pasteur de Lille, France
  • 3Department of Medicine, McMaster University, Hamilton, Ontario, Canada
Further Information

Publication History

Received 23 March 2005

Accepted after revision 10 August 2005

Publication Date:
13 February 2006 (online)

Abstract

Adipocytes produce the endothelial-cell specific molecule-1 (ESM-1), which inhibits leukocyte adhesion and migration through the endothelium. This study investigates ESM-1 expression and regulation in human adipose tissue. Subcutaneous abdominal adipose tissue was obtained from seventy postmenopausal women. Fourteen women subsequently underwent non-pharmacological weight reduction. In vitro experiments were performed on adipocytes isolated from human mammary adipose tissue. We determined gene expression by TaqMan RT-PCR and measured ESM-1 levels in serum and cell culture medium by ELISA. Mature adipocytes produced ESM-1. ESM-1 gene expression was higher in adipocytes than in preadipocytes. Cortisol inhibited ESM-1 gene expression in preadipocytes. Insulin and cortisol inhibited adipocyte ESM-1 production in adipocytes. This inhibitory effect of insulin was attenuated by insulin resistance, as ESM-1 gene expression in subcutaneous adipose tissue was increased in obese, hyperinsulinemic women. In contrast, ESM-1 serum levels were reduced in obese women and inversely correlated to C-reactive protein levels. Five percent weight loss did not markedly change gene expression. Circulating ESM-1 levels increased significantly, albeit modestly. ESM-1 is actively produced by adipocytes. However, since ESM-1 adipocyte gene expression and circulating plasma levels are not correlated, other sources of ESM-1 may be more important. Circulating ESM-1 levels are reduced in the overweight and obese, consistent with the notion that ESM-1 may play some role in obesity-associated vascular disease.

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Jürgen Janke, Ph. D.

Franz-Volhard Klinik · Charité

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Fax: +49 30 9417 2206

Email: janke@fvk.charite-buch.de