Abstract
Investigation of gene expression is a developing area with several methods available. One method is quantitative PCR. A major pitfall in quantitative PCR is the normalisation procedure of the gene expression. Many experiments include a housekeeping gene, some use RNA concentration, and others use a geometric mean of several internal, stably expressed genes. This study demonstrates that real-time-PCR results differ with varying housekeeping genes and analysis protocols when applied to insulin-secreting INS-1E cells derived from the pancreas and stimulated by DEDTC (diethyldithiocarbamate, a zinc chelator) and GLP-1.
Key words
DEDTC - GLP-1 - UBC-7 - HPRT - cyclophilin A - ZnT-3 - ZnT-8
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Jørgen Rungby
Department of Clinical Pharmacology · University of Aarhus · Denmark ·
Phone: +45 8942 1708
Fax: +45 8612 8804
Email: jr@farm.au.dk
