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DOI: 10.1055/s-2005-916228
Differentiation of Dendrobium Species used as ”Huangcao Shihu” by rDNA ITS Sequence Analysis
Publication History
Received: March 25, 2005
Accepted: June 1, 2005
Publication Date:
05 December 2005 (online)
![](https://www.thieme-connect.de/media/plantamedica/200601/lookinside/thumbnails/10.1055-s-2005-916228-1.jpg)
Abstract
The genus Dendrobium Sw. is composed of 74 species and two varieties in China, and 32 species carry the name ”Huangcao Shihu” on the herbal medicine market, making the identification of the origin of ”Huangcao Shihu” difficult for consumers. Here, the ITS regions were sequenced and evaluated to differentiate the 18 Dendrobium species used as ”Huangcao Shihu”. Diversity in DNA sequences among various species was found with the inter-specific sequence divergence ranging from 3.2 % to 37.9 % in ITS1 and 5.0 % to 26.6 % in ITS2. Moreover, the variations within species were very low, ranging in sequence divergence from 0 to 3.0 % in ITS1 and 0 to 4.0 % in ITS2. Therefore, these species could be easily distinguished at the DNA level. Furthermore, based on the divergent ITS regions, five pairs of species-specific primers were designed and used for the rapid PCR identification of five Dendrobium species listed in the Chinese Pharmacopoeia.
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Prof. Zhengtao Wang
Institute of Chinese Materia Medica
Shanghai University of Traditional Chinese Medicine
Shanghai 201203
People’s Republic of China
Fax: +86-21-5132-2519
Email: wangzht@hotmail.com