Planta Med 2006; 72(2): 136-142
DOI: 10.1055/s-2005-873191
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Anti-Inflammatory Activity of 5-O-Demethylnobiletin, a Polymethoxyflavone Isolated from Sideritis tragoriganum

Esperanza Bas1 , M. Carmen Recio1 , Rosa M. Giner1 , Salvador Máñez1 , Miguel Cerdá-Nicolás2 , José-Luis Ríos1
  • 1Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Valencia, Spain
  • 2Departament de Patologia, Facultat de Medicina, Universitat de València, Valencia, Spain
Further Information

Publication History

Received: April 8, 2005

Accepted: July 13, 2005

Publication Date:
10 November 2005 (online)

Abstract

We have studied the effect of 5-O-demethylnobiletin (1) on both the inflammation of mouse ears induced by repeated application of 12-O-tetradecanoylphorbol 13-acetate (TPA) and the acute mouse paw oedemas induced by carrageenan and phospholipase A2 (PLA2), and determined its activity on 5-lipoxygenase (5-LOX) and elastase release/activity. Compound 1 reduced the oedema formation, cell infiltration, and tissue damage in the inflammation induced by TPA in mouse ears, along with the acute oedema induced by carrageenan in mouse paws and the acute PLA2-induced oedema in mouse paws. The flavone inhibited leukotriene B4 formation in rat neutrophils and elastase release in human neutrophils, but did not reduce the expression of cyclooxygenase-2 (COX-2) in murine RAW 264.7 macrophages. These experimental results suggest that 1 may act through a direct inhibition of 5-LOX, without affecting the expression of COX-2.

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Prof. Dr. José Luis Ríos

Departament de Farmacologia

Facultat de Farmàcia

Universitat de València

Av. Vicent Andrés Estellés s/n

46100-Burjassot

Spain

Phone: +34-963-544-973

Fax: +34-963-544-973

Email: riosjl@uv.es