Horm Metab Res 2005; 37(5): 275-280
DOI: 10.1055/s-2005-861469
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Participation of Protein Kinases in the Stimulant Action of GLP-1 on 2-deoxy-D-glucose Uptake by Normal Rat Skeletal Muscle

A.  Acitores1 , N.  González1 , V.  Sancho1 , L.  Arnés1 , I.  Valverde1 , W.  J.  Malaisse2 , M.  L.  Villanueva-Peñacarrillo1
  • 1 Department of Metabolism, Nutrition and Metabolism, Fundación Jiménez Díaz, Madrid, Spain
  • 2 Laboratory of Experimental Hormonology, Brussels Free University, Brussels, Belgium
Further Information

Publication History

Received 22 November 2004

Accepted after revision 17 February 2005

Publication Date:
22 June 2005 (online)

Abstract

Several protein kinases were recently proposed for involvement in GLP-1-stimulated D-glucose transport in skeletal muscle from both normal subjects and type 2 diabetic patients. This study was mainly aimed at investigating the effect of potential inhibitors of distinct protein kinases and protein phosphatase-1 upon insulin- and GLP-1-stimulated 2-deoxy-D-glucose net uptake by normal rat skeletal muscle. The basal uptake of the D-glucose analog was decreased by wortmannin - a phosphatidylinositol-3-kinase inhibitor -, PD98059 - a mitogen-activated protein kinases inhibitor -, and TNFα - a protein phosphatase-1 inhibitor -, but not by either rapamycin - a p70s6 kinase inhibitor -, or H-7 -, a protein kinase C inhibitor -. The enhancing action of both insulin and GLP-1 upon 2-deoxy-D-glucose transport was abolished by PD98059 and H-7, but largely unaffected by TNFα. Wortmannin and rapamycin preferentially affected the response to GLP-1 and insulin, respectively. These findings thus document both analogies and dissimilarities in the participation of the concerned enzymes in the stimulant action of insulin versus GLP-1 upon D-glucose transport in normal rat skeletal muscle.

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M. L. Villanueva-Peñacarrillo, Ph. D.

Dpt. Metabolismo · Nutrición y Hormonas · Fundación Jiménez Díaz · Avda

Reyes Católicos · 2 · 28040 Madrid · Spain

Email: mlvillanueva@fjd.es