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DOI: 10.1055/s-2005-837817
© Georg Thieme Verlag KG Stuttgart · New York
Protopanaxatriol-Type Ginsenosides Differentially Modulate Type 1 and Type 2 Cytokines Production from Murine Splenocytes
Publication History
Received: May 30, 2004
Accepted: October 30, 2004
Publication Date:
15 March 2005 (online)
![](https://www.thieme-connect.de/media/plantamedica/200503/lookinside/thumbnails/10.1055-s-2005-837817-1.jpg)
Abstract
Seven protopanaxatriol-type ginsenosides and their aglycones including PPT, PT, -Re, -Rg1, -F1, -Rh1, 20(R)-Rh1 which are closely related in structure were studied for their effects on type 1 and type 2 cytokines production from murine splenocytes and their related mechanisms were examined. The results indicate that PPT, PT and ginsenoside-Re show hardly any or weak effects on concanavalin A (Con A)-induced production of IFN-γ and IL-4. Ginsenoside-Rh1 and 20(R)-Rh1 induce a Con A-induced type 1 cytokine pattern by increasing the production of interleukin-12 (IL-12), the expression of IFN-γ, T-bet and enhancing NF-κB DNA binding activity. In contrast ginsenosides-Rg1 and -F1 cause a Con A-induced type 2 cytokines response by increasing the expression of IL-4, GATA-3 and enhancing NF-κB DNA binding activity. Thus, these protopanaxatriol-type ginsenosides have different immunomodulatory effects, which might explain the complex immunomodulatory effect of Panax ginseng.
Key words
Ginsenosides - interferon-γ (IFN-γ) - interleukin-4 (IL-4) - t-box expressed in T cells (T-bet) - GATA-binding protein 3 (GATA-3) - splenocytes - Panax ginseng - Araliaceae
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Gui-Fang Cheng
Department of Pharmacology
Institute of Materia Medica
Chinese Academy of Medical Sciences and Peking Union Medical College
1 Xian Nong Tan Street
Xuan Wu District
100050, Beijing
People′s Republic of China
Phone: +86-10-6316-5192
Fax: +86-10-6301-7757
Email: chenggf@imm.ac.cn