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DOI: 10.1055/s-2005-837787
© Georg Thieme Verlag KG Stuttgart · New York
Miniaturization of the Structure Elucidation of Novel Natural Products - Two Trace Antibacterial Acylated Caprylic Alcohol Glycosides from Arctostaphylos pumila
Publication History
Received: May 17, 2004
Accepted: August 29, 2004
Publication Date:
24 February 2005 (online)
![](https://www.thieme-connect.de/media/plantamedica/200502/lookinside/thumbnails/10.1055-s-2005-837787-1.jpg)
Abstract
High-throughput isolation, purification and analysis methods applied to natural products libraries from plants gave rise to the discovery of two novel acylated caprylic alcohol glycosides (1, 2) produced by Arctostaphylos pumila. The NMR spectra were acquired using the CapNMR™ probe and performed on mass-limited samples, which enabled us to elucidate the structures of 2,6-diacetyl-3,4-diisobutyl-1-O-octylglucopyranoside (1, 200 μg) and 2,6-diacetyl-3,4-dimethylbutyl-1-O-octylglucopyranosid (2, 70 μg). Compounds 1 and 2 exhibited antibacterial activity against Gram-positive methicillin-resistant Staphylococcus aureus with an MIC of 128 μg/mL and 64 μg/mL, respectively.
Key words
High-throughput natural products chemistry - CapNMRTM probe - Arctostaphylos pumila - Ericaceae - acylated caprylic alcohol glycosides - antibacterial.
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Dr. Jin-Feng Hu
Lead Discovery & Rapid Structure Elucidation Group
Sequoia Sciences, Inc.
11199 Sorrento Valley Road, Suite H
San Diego
CA 92121
USA
Fax: +1-858-623-0805
Email: jhu@sequoiasciences.com