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DOI: 10.1055/s-2005-837465
© Georg Thieme Verlag KG Stuttgart · New York
Possible Interactions between Antioxidant Enzymes and Free Sialic Acids in Saliva: A Preliminary Study on Elite Judoists
Publikationsverlauf
Accepted after revision: November 25, 2004
Publikationsdatum:
22. Februar 2005 (online)
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Abstract
Measurement of antioxidant enzyme activities is of great importance in the evaluation of oxidative stress in human metabolism. Sialic acids are also vital bio-markers for some diseases such as acute myocardial infarction and diabetes. In the present study, antioxidant enzyme activities (SOD, CAT, GSH-Px) and free sialic acid (FSA) levels in saliva were determined before and after training in the elite Turkish judoists (ETJ). According to the results, antioxidant enzyme activities in post-exercise values were significantly (p < 0.05) higher than those of pre-exercise values. A major finding of this study was that FSA levels also increased significantly (p < 0.05). Superoxide radical anion might have been effectively dismutated to hydrogen peroxide by elevated SOD activity. Accordingly, it suggests that CAT, having a higher increasing rate, was more efficient than GSH-Px in decomposition of hydrogen peroxide. In conclusion, the increase in antioxidant enzyme activities might have caused the elevation of FSA levels after training. Over-excreted sialic acids to saliva might have an important role in the removal of hydrogen peroxide. Since the increase in FSA levels in saliva has been found to be in well accordance with antioxidant enzymes, FSA may be concluded as an alternative oxidative stress marker in athletes.
Key words
Antioxidant enzymes - judo - physical exercise - sialic acid
References
-
1 Aebi H.
Catalase. Packer L Methods in Enzymology. San Diego; Academic Press 1985: 121-126 - 2 Alessio H M, Blasi E R. Physical activity as a natural antioxidant booster and its effects on a healthy life span. Res Q Exer Sport. 1997; 68 292-302
- 3 Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72 248-254
- 4 Brown D W, Brown D R, Heath G W, Balluz L, Giles W H, Ford E S, Mokhad A H. Associations between physical activity dose and health-related quality of life. Med Sci Sports Exerc. 2004; 36 890-896
- 5 Cavas L. Does underwater rugby stimulate the over-production of reactive oxygen species?. Cell Biochem Func. 2005; 23 59-63
- 6 Cavas L, Tarhan L. Effects of vitamin-mineral supplementation on cardiac marker and radical scavenging enzymes, and MDA levels in young swimmers. Int J Sport Nut Exerc Met. 2004; 14 133-146
- 7 Cavas L, Tarhan L. The relationship between total sialic acid levels and antioxidant status in the tadpoles of Bufo viridis and Rana ridibunda ridibunda. Cell Biochem Funct. 2003; 21 257-261
- 8 Crosti N, Servidei T, Bajer J, Serra A. Modification of the 6-hydroxydopamine technique for the correct determination of superoxide dismutase. Clin Chem Clin Biochem. 1987; 25 265-266
- 9 Halliwel B, Gutteridge (ed) J MC. Free Radicals in Biology and Medicine. New York; Oxford Uni Press 1998
- 10 Husain K. Interaction of physical training and chronic nitroglycerin treatment on blood pressure, nitric oxide, and oxidants/antioxidants in the rat heart. Pharmacol Res. 2003; 48 253-261
- 11 Iijima R, Takahashi H, Namme R, Ikegami S, Yamazaki M. Novel biological function of sialic acid (N-acetylneuraminic acid) as a hydrogen peroxide scavenger. FEBS Lett. 2004; 561 163-166
- 12 Javors M A, Johnson B A. Current status of carbohydrate deficient transferrin, total serum sialic acid, sialic acid index of apolipoprotein J and serum beta-hexosaminidase as markers for alcohol consumption. Addiction. 2003; 98 S45-S50
- 13 Jenkins R R. Free radical chemistry. Relationship to exercise. Sports Med. 1988; 5 156-170
- 14 Jennings G LR. Mechanism for reduction of cardiovascular risk by regular exercise. Clin Exp Pharmacol Physiol. 1995; 22 209-211
- 15 Kakari S, Stringou E, Toumbis M. Five tumor markers in lung cancer: Significance of total and lipid bound sialic acid. Anticancer Res. 1991; 11 2107-2110
- 16 Lukaski H C, Hoverson B S, Gallagher S K, Bolonchuk W W. Physical training and copper, iron, and zinc status of swimmers. Am J Clin Nut. 1990; 51 1093-1099
- 17 Metin G, Atukeren P, Alturfan A A, Gulyasar T, Kaya M, Gumustas M K. Lipid peroxidation, erythrocyte superoxide-dismutase activity and trace metals in young male footballers. Yonsei Med J. 2003; 44 979-986
- 18 Paglia D E, Valentine W N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70 158-169
- 19 Painbeni T, Gamelin E, Cailleux A, Le Bouil A, Boisdron-Celle M, Daver A, Larra F, Allain P. Plasma sialic acid as a marker of the effect of the treatment on metastatic colorectal cancer. Eur J Cancer. 1997; 33 2216-2220
- 20 Schauer R. Sialic acids. Trends Biochem Sci. 1985; 7 357-360
- 21 Sprague E A, Moser M, Edwards E H, Schwartz C J. Stimulation receptor mediated low density lipoprotein endocytosis in neuraminidase-treated cultured bovine aortic endothelial cells. J Cell Physiol. 1998; 137 251-262
- 22 Spyridaki M HE, Siskos P A. An improved spectrophotometric method for the determination of free, bound and total N-acetylneuraminic acid in biological fluids. Anal Chim Acta. 1996; 327 277-285
- 23 Suer-Gokmen S, Kilicli G, Ozcelik F, Ture M, Gulen S. Association between serum total and lipid bound sialic acid concentration and the severity of coronary atherosclerosis. J Lab Clin Med. 2002; 140 110-118
- 24 Tauler P, Aguilo A, Gimeno I, Fuentespina E, Tur J A, Pons A. Influence of vitamin C diet supplementation on endogenous antioxidant defences during exhaustive exercise. Pflugers Arch. 2003; 446 658-664
- 25 Tiidus P M, Pushkarenko J, Houston M E. Lack of antioxidant adaptation to short-term aerobic training in human muscle. Am J Physiol. 1996; 271 R832-R836
- 26 Urso M L, Clarkson P M. Oxidative stress, exercise, and antioxidant supplementation. Toxicology. 2003; 189 41-54
- 27 Vimr E R, Kalivoda K A, Deszo E L, Steenbergen S M. Diversity of microbial sialic acid metabolism. Microbiol Mol Biol Rev. 2004; 68 132-153
- 28 Wendel-Vos G CW, Schuit A J, Tijhuis M AR, Kromhout D. Leisure time physical activity and health-related quality of life: Cross-sectional and longitudinal associations. Qual Life Res. 2004; 13 667-677
L. Cavas
Dokuz Eylül University, Faculty of Arts and Sciences, Department of Chemistry (Biochemistry Division)
Kaynaklar Campus
35160 Izmir
Turkey
Telefon: + 902324535074-2138
Fax: + 90 23 24 53 41 88
eMail: lcavas@deu.edu.tr