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DOI: 10.1055/s-2004-832651
© Georg Thieme Verlag KG Stuttgart · New York
Phylogenetic Analysis of the DNA Sequence of the Non-Coding Region of Nuclear Ribosomal DNA and Chloroplast of Ephedra Plants in China
Publication History
Received: February 6, 2004
Accepted: March 14, 2004
Publication Date:
18 November 2004 (online)
![](https://www.thieme-connect.de/media/plantamedica/200411/lookinside/thumbnails/10.1055-s-2004-832651-1.jpg)
Abstract
Twenty-four Ephedra plants belonging to 8 species grown in the northern and western parts of China were phylogenetically analyzed for their non-coding DNA sequences, internal transcribed spacers (ITSs) of nuclear ribosomal DNA as well as trnL intron and intergenic spacers between trnL and trnF (trnL/trnF) of the chloroplast. Based on the ITS sequences, the 8 species could be divided into 3 groups: Group 1 (Ephedra intermedia, E. sinica, E. przewalskii), Group 2 (E. equisetina, E. monosperma, E. gerardiana), and Group 3 (E. likiangensis, E. minuta). The species classified into Group 1 grow mainly in the north, Group 3 in the south and Group 2 in the center, suggesting their genetic and geographic relationships. A specific primer set was designed to classify the 3 groups by routine PCR. Combined analysis of ITS and trnL/trnF differentiated the 8 Ephedra species.
Abbreviations
ITS1:internal transcribed spacer 1
ITS2:internal transcribed spacer 2
trnL/trnF:intron of trnL and intergenic spacer between trnL and trnF
EI:Ephedra intermedia
ES:Ephedra sinica
EP:Ephedra przewalskii
EE:Ephedra equisetina
EM:Ephedra monosperma
EG:Ephedra gerardiana
EL:Ephedra likiangensis
EMu:Ephedra minuta
Key words
Ephedra - Ephedraceae - nuclear ribosomal DNA - internal transcribed spacer (ITS) - chloroplast DNA - intron of trnL and intergenic spacer between trnL and trnF (trnL/trnF)
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Dr. N. Kakiuchi
Faculty of Pharmaceutical Sciences
Kanazawa University
13-1 Takaramachi
Kanazawa 920-0934
Japan
Fax: +81-76-234-4490
Email: kakiuchi@p.kanazawa-u.ac.jp