Immunohistochemical Detection of Specific S100a13 Immunoreactivity in Middle Ear Cholesteatoma

Objective:

To conduct a comparative morphologic study on expression of S100a13, a novel member of Ca²⁺-binding proteins superfamily, in middle ear cholesteatoma and meatal skin.

Material and Methods:

5 middle ear cholesteatoma samples and the corresponding external auditory canal skin specimens were subjected to indirect immunofluorescence using a polyclonal monospecific anti-S100a13 serum, developed in our laboratory. Laryngeal carcinoma samples were used as a positive control in this study.

Results:

Specific S100? 13 immunoreactivity was revealed in all layers of the cholesteatoma matrix and subepithelial granulation tissue. No specific flurescence for S100a13 was detected in the meatal skin epithelium. Elevated levels of expression of S100a13 were disclosed only in dermal layer of the external auditory skin samples.

Conclusions:

Our findings suggest that S100a13 is exclusively expressed in middle ear cholesteatoma matrix, which give us ground to conclude that this marker could be related to the hyperproliferative properties of cholesteatoma tissue and the accompanying neo- angiogenesis.

Key words

S100a13 - angiogenesis - cholesteatoma - hyperproliferation