RSS-Feed abonnieren
DOI: 10.1055/s-2004-818971
© Georg Thieme Verlag KG Stuttgart · New York
Stability Control of Senna Leaves and Senna extracts
Publikationsverlauf
Received: October 27, 2003
Accepted: January 30, 2004
Publikationsdatum:
04. Mai 2004 (online)
![](https://www.thieme-connect.de/media/plantamedica/200405/lookinside/thumbnails/10.1055-s-2004-818971-1.jpg)
Abstract
Powdered senna leaves and a commercial methanolic senna leaf extract were investigated for apparent degradation pathways of known constituents. Different defined storage conditions were chosen according to the guidelines of the international conference on harmonization. Analytical fingerprinting was carried out by HPLC with photodiode array detection. Differences in degradation pathways were observed between the powdered herbal drug material and the extract, depending on storage conditions and packaging materials. Within the crude plant material sennosides were shown to be degraded to sennidine monoglycosides, while rhein 8-O-glucoside was hydrolysed to rhein by enzymatic processes. Degradation of the anthranoid compounds was not due to the same pathways in the investigated commercial extracts. Only unspecific alterations of all compounds were observed. Forced decomposition of this herbal drug preparation under high temperature caused oxidative decomposition of the sennosides to rhein 8-O-glucoside. Furthermore flavonoid glycosides decomposition were observed with an apparent increase in the content of flavone aglyca.
Key words
Stability control - sennosides - degradation pathways - Cassia senna - Leguminosae - ICH guidelines
References
- 1 CPMP (Committee for proprietary medicinal p roducts). Note for guidance on quality of herbal medicinal products. 2001: CPMP/QWP/2819/00
- 2 Bakshi M, Saranjit S. Development of validated stability-indicating assay methods - critical review. J Pharm Biomed Anal. 2002; 28 1011-40
- 3 CPM P. Note for guidance on stability testing of existing active substances and related finished products. 1998: CPMP/ICH/556/96
- 4 Lazarowych N, Pekos P. Use of fingerprinting and marker compounds for identification and standardization of botanical drugs: strategies for applying pharmaceutical HPLC analysis to herbal products. Drug Inf J. 1998; 32 497-512
- 5 Terreaux T, Wang Q, Loset J R, Ndjoko K, Grimminger W, Hostettmann K. Complete LC/MS analysis of a Tinnevelli Senna pod extract and subsequent isolation and identification of two new benzophenone glucosides. Planta Med. 2002; 68 349-54
- 6 Bala S, Uniyal G C, Dubey T, Singh S P. An improved method for the analysis of sennosides in Cassia angustifolia by high-performance liquid chromatography. Phytochem Anal. 2001; 12 277-80
- 7 Metzger W, Reif K. Determination of 1,8-dihydroxyanthranoids in senna. J Chromatogr A. 1996; 740 133-8
- 8 Kinjo J, Ikeda T, Watanabe K, Nohara T. An anthraquinone glycoside from Cassia angustifolia leaves. Phytochemistry. 1994; 37 1685-7
- 9 Heigl D, Franz G. Stability testing on typical flavonoid containing herbal drugs. Pharmazie. 2003; 58 881-5
- 10 Meilhammer B. Untersuchungen zur Hydroxyanthracenfreisetzung aus Abführtees unter haushaltsnahen Extraktionsbedingungen. PhD Thesis Regensburg; 2003
Prof. Dr. G. Franz
Department of Pharmaceutical Biology
Institute of Pharmacy
University of Regensburg
Universitätsstrasse 31
93040 Regensburg
Germany
Telefon: +49-941-943-4761
Fax: +49-941-943-4762
eMail: Gerhard.franz@chemie.uni-regensburg.de