Planta Med 2004; 70(3): 256-258
DOI: 10.1055/s-2004-815544
Letter
© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effects of ent-Kauranes from the Stems of Annona squamosa on Superoxide Anion Generation by Human Neutrophils

Yu-Liang Yang1 , Fang-Rong Chang1 , Tsong-Long Hwang2 , Wen-Te Chang2 , Yang-Chang Wu1
  • 1Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C.
  • 2Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan, R.O.C.
This investigation was supported by a grant from the National Science Council of Republic of China
Further Information

Publication History

Received: August 25, 2003

Accepted: November 29, 2003

Publication Date:
23 March 2004 (online)

Abstract

Eleven ent-kauranes, isolated from the fresh stems of Annona squamosa L. (Annonaceae), were subjected to assays on the generation of superoxide anion (O2 ·-) by human neutrophils. Except for ent-kaur-16-en-19-oic acid, 16β,17-dihydroxy-ent-kauran-19-al, and 16α,17-dihydroxy-ent-kauran-19-al, all ent-kauranes showed significant inhibitory effect on O2 ·- generation in response to formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP/CB). In contrast, phorbol myristate acetate (PMA)-induced O2 ·- generation was not suppressed by any ent-kauranes. Especially, ent-kaur-16-en-19-oic acid could significantly increase O2 ·- production. The structure-activity relationship of these compounds is also discussed herein. Furthermore, the effect of ent-kauranes on nitric oxide generation by NR8383 macrophages in response to lipopolysaccharide (LPS) was examined. None of the compounds showed an inhibitory effect on nitric oxide generation.

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Professor Yang-Chang Wu

Graduate Institute of Natural Products

Kaohsiung Medical University

Kaohsiung 807

Taiwan

Republic of China

Phone: +886-7-3121101 ext. 2197

Fax: +886-7-3114773

Email: yachwu@kmu.edu.tw