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DOI: 10.1055/s-2003-818014
Quantitative Analysis of Strychnine and Brucine in Strychnos nux-vomica using 1H-NMR
The Post-doctoral Fellowship Program of the Korea Science Engineering Foundation (KOSEF) and the Belgian National Fund for Scientific Research (FNRS) are gratefully acknowledged for their support to of Y.H. Choi and M. Frédérich, respectivelyPublication History
Received: May 7, 2003
Accepted: September 7, 2003
Publication Date:
29 January 2004 (online)
Abstract
A quantitative analysis using 1H-NMR (Q-NMR) has been developed for the determination of strychnine and brucine in Strychnos nux-vomica seeds and stems. The advantages of the method are that no reference alkaloids are needed for calibration curves, the quantification could be directly realized on a crude extract, strychnine and brucine could easily be distinguished, an overall profile of the preparation (including non alkaloid compounds) could be directly obtained, and a very significant time-gain could be achieved, in comparison to conventional HPLC methods, for instance.
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Robert Verpoorte
Division of Pharmacognosy
Leiden University
Institute of Biology
Section Metabolomics
P.O. Box 9502
2300 RA Leiden
The Netherlands
Fax: +31-71-527-4503
Email: Verpoort@chem.leidenuniv.nl