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DOI: 10.1055/s-2003-818008
Demethylbellidifolin Inhibits Adhesion of Monocytes to Endothelial Cells via Reduction of Tumor Necrosis Factor alpha and Endogenous Nitric Oxide Synthase Inhibitor Level
This study was supported by a grant from the Provincial Natural Science Foundation of Hunan, China, No. 02jjy2046Publication History
Received: May 7, 2003
Accepted: August 30, 2003
Publication Date:
29 January 2004 (online)
![](https://www.thieme-connect.de/media/plantamedica/200312/lookinside/thumbnails/10.1055-s-2003-818008-1.jpg)
Abstract
The effect of demethylbellidifolin (DMB), a major compound of Swertia davidi Franch, on the adhesion of monocytes to endothelial cells induced by oxidized low-density lipoprotein (ox-LDL) was studied. Adhesion of monocytes to endothelial cells was induced by treatment with ox-LDL (100 μg/mL) for 48 h. Levels of tumor necrosis factor-α (TNF-α) and asymmetric dimethylarginine (ADMA, an endogenous inhibitor of NOS) in conditioned medium and the activity of dimethylarginine dimethylaminohydrolase (DDAH) in endothelial cells were measured. DMB (3 or 10 μmol/L) significantly inhibited the adhesion of monocytes to endothelial cells, attenuated an increase in levels of TNF-α and ADMA, and a decrease in the activity of DDAH by ox-LDL. The present results suggest that DMB inhibits the increased adhesion of monocytes to endothelial cells induced by ox-LDL, and that the effect of DMB is related to reduction of the ADMA concentration via reduction of TNF-α production in cultured endothelial cells treated with ox-LDL.
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Yuan-Jian Li, MD
Department of Pharmacology
School of Pharmaceutical Sciences
Central South University
Xiang-Ya Road #88
Changsha 410078
P. R. China
Phone: +86-731-4805441
Fax: +86-731-2650442
Email: yuan_jianli@yahoo.com