Gonadotropin-Releasing Hormone Increases Melatonin Release in the   Pineal Gland of the Female Rat In Vitro

M. T. Itoh; T. Hosaka; T. Mimuro; N. Hamada; B. Ishizuka

doi:10.1055/s-2003-39076

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Horm Metab Res 2003; 35(3): 153-157
DOI: 10.1055/s-2003-39076

Original Basic

Gonadotropin-Releasing Hormone Increases Melatonin Release in the Pineal Gland of the Female Rat In Vitro

M. T. Itoh ¹ , T. Hosaka ² , T. Mimuro ² , N. Hamada ² , B. Ishizuka ²

¹ Department of Chemistry, St. Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki, Japan
² Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki, Japan

Further Information

Publication History

Received 2 July 2002

Accepted after revision 8 October 2002

Publication Date:
07 May 2003 (online)

Also available at

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Abstract

To evaluate the effect of gonadotropin-releasing hormone (GnRH) on melatonin (N-acetyl-5-methoxytryptamine) release and its synthesizing enzyme activities in pineal glands, pineals from adult female rats during diestrus were organ-cultured in a medium containing 10^-12, 10^-10, or 10^-8 M GnRH for 6 h. Melatonin release increased significantly in pineals cultured with 10^-10 and 10^-8 M GnRH compared to controls. However, in pineal glands that were organ-cultured in a medium containing 10^-12 to 10^-8 M GnRH, the activity of arylalkylamine N-acetyltransferase, which is the key regulatory enzyme in melatonin biosynthesis, showed no significant difference from controls. Likewise, GnRH at these concentrations had no significant effect on the activity of pineal hydroxyindole-O-methyltransferase, which catalyzes the final step of melatonin biosynthesis. These results show that GnRH stimulates pineal melatonin release, but suggest that GnRH does not affect its melatonin synthesis.

Key words

Melatonin - Arylalkylamine N-Acetyltransferase - Hydroxyindole-O-Methyltransferase - Gonadotropin-Releasing Hormone - Pineal Gland - Organ Culture - Female Rats

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M. T. Itoh, Ph. D.

Department of Chemistry · St. Marianna University School of Medicine

Sugao · Miyamae-ku · Kawasaki 216-8511 · Japan

Phone: +81 (44) 977-8111 ext. 4517

Fax: + 81 (44) 977-8133

Email: itohmt@marianna-u.ac.jp