We investigated the effect of an acute bout of endurance exercise on
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untrained rats. Fifty rats were equally divided into a trained and an untrained
group. Rats of the trained group ran on a treadmill 45 min/day for 5
days. On the sixth day, 5 rats were killed without exercise, while the
remaining 20 ran as above and were killed 0, 3, 6, and 12 h
post-exercise (5 rats at each time point). In the untrained group, 5 rats were
killed without exercise, while the remaining 20 ran as above only once and were
killed at the same time points as the trained group. Western blotting
demonstrated no significant changes in c-Fos protein levels in the untrained
group. On the contrary, in the trained group, there was a significant increase
at 6 and 12 h compared to 3 h post-exercise. The levels of the
protein in the trained rats were above the corresponding levels in the
untrained ones at all time points, although these differences reached
statistical significance only immediately, 6 h and 12 h
post-exercise. These results show that trained skeletal muscle exhibits
increased levels of c-Fos, probably as a cumulative result of changes occurring
during recovery from each exercise bout, and greater c-Fos response after acute
endurance exercise compared to untrained skeletal muscle.
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