In Vitro Cytotoxic Elemanolides from Vernonia lasiopus

Abstract

Two new elemanolides, epivernodalol and lasiopulide, were isolated after chromatographic separation of the alcoholic extract of the dried aerial parts of the Vernonia lasiopus. These elemanolides are new C-10 epimers of the sesquiterpene lactones vernodalol and demethylacroylated vernodalol isolated from other species of Vernonia. Both elemanolides showed in vitro cytotoxicity against human cancer cell lines in culture. This is the first report of isolation and cytotoxic activity of the two elemanolides from V. lasiopus.

References

• 1 Hedberg I, Hedberg O, Madati P S, Mshigeni K E, Mshiu E N, Samuelsson G. Inventory of plants used in traditional medicine in Tanzania-1. Plants of the family Acanthaceae, Cucurbitaceae.  J Ethnopharmacol. 1982;  6 29-60
• 2 Perdue RE J r, Carlson K D, Gillbert M G. Vernonia galamensis, Potential new crop source of epoxy acid.  Economic Botany. 1986;  40 54-68
• 3 Bingel A S, Fransworth N R. Higher plants as potential sources of galactagogues. In: Wagner H, Fransworth NR, editors Economic and Medicinal Plant Research. Academic Press Limited London; 1994: pp 1-48
• 4 Kupchan S M, Hemingway R J, Werner D, Karim A. Tumor inhibitors. XLVI. Vernolepin, a novel sesquiterpene dilactone tumor inhibitor from Vernonia hymenolepis A. Rich.  J Org Chem. 1969;  34 3903-8
• 5 Kupchan S M, Hemingway R J, Karim A, Werner D. Tumor inhibitor. XLVII. Vernodalin and vernomygdin, two new cytotoxic sesquiterpene lactones from Vernonia amygdalina Del.  J Org Chem. 1969;  34 3908-11
• 6 Ohta Y, Andersen N H, Liu C B. Sesquiterpene constituents of two liverworts of genus Diplophyllum, Novel eudesmanolides and cytotoxicity studies for enantiomeric methylene lactones.  Tetrahedron. 1977;  33 617-28
• 7 Asakawa Y. Biologically active substances from Bryophytes. In: Chopra RN, Bhatia SC, editors Bryophyte development physiology and biochemistry. Boca Raton; CRC Press 1990: p 259
• 8 Asakawa Y. Terpenoids and aromatic compounds with pharamacological activity from Brophytes. In: Zinsmeister HD, Mues R, editors Bryophytes: Their chemistry and chemical taxonomy. Oxford; Oxford University Press 1990: p 369
• 9 Bohlmann F, Jakupovic J. Progress in the chemistry of the Vernonieae (Compositae).  Pl Syst Evol. 1990;  [Suppl 4] pp 3-43
• 10 Harborne J B, Williams C A. Vernonieae-chemical review. In: Heywood VH, Harborne JB, Turner BL, editors The biology and chemistry of the Compositae. Vol. 1 Academic Press London; 1977: p 523
• 11 Johri R K, Singh C. Medicinal uses of Vernonia species.  J Med Arom Plant Sci. 1997;  19 744-52
• 12 Johri R K, Singh C, Koul B L. V. lasiopus and V. galamensis: A medicinal perspective.  Res Ind. 1995;  40 327-8
• 13 Asaka Y, Kubota T, Kulkarni A B. Studies on a bitter principle from Vernonia anthelmintica .  Phytochemistry. 1977;  16 1838-9
• 14 Singh C, Ram G, Thakur R N, Koul B L. Vernonia species renewable plant sources of epoxy oil for industrial use.  J Econ Tax Bot. 1994;  18 501-13
• 15 Monks A, Scudiero D, Skehan P, Shoemaker R, Paull K, Vistica D. et al . Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumour cell lines.  J Natl Cancer Inst. 1991;  83 757-66