Endoscopy 2003; 35(3): 197-202
DOI: 10.1055/s-2003-37270
Original Article
© Georg Thieme Verlag Stuttgart · New York

Standardized Reprocessing of Reusable Colonoscopy Biopsy Forceps is Effective: Results of a German Multicenter Study

M.  Jung 1 , U.  Beilenhoff 1 , M.  Pietsch 2 , B.  Kraft 2 , G.  Rippin 3
  • 1St. Hildegardis Hospital, Mainz, Germany
  • 2Dept. of Hygiene and Environmental Medicine, Johannes-Gutenberg-Universität, Mainz, Germany
  • 3Institute of Medical Statistics, University of Mainz, Mainz, Germany
Further Information

Publication History

Submitted: 24 October 2002

Accepted after Revision: 16 December 2002

Publication Date:
13 February 2003 (online)

Background and Study Aims: National and international guidelines recommend that a standardized protocol consisting of cleaning, ultrasound cleaning, and sterilization should be used for the reprocessing of endoscopic accessories in order to reduce the risk of transmission of microorganisms. This German multicenter study investigated the efficacy of standardized reprocessing of reusable biopsy forceps used during colonoscopy.
Materials and Methods: Ten endoscopy centers (eight hospitals and two private practices) used 330 biopsy forceps during routine colonoscopy. The forceps were used once, five times, or 20 times for colonoscopy, based on a randomization plan. The reprocessing protocol consisted of manual cleaning with an enzymatic agent, ultrasound cleaning with an enzymatic agent (30 min, 40 °C, 47 Hz), neutralization, drying, and sterilization (5 min, 134 °C). Aldehydes were not used, and the protocol did not include a disinfection step. The biopsy forceps were sent to three microbiological institutes, based on a randomization plan, to have them tested for the presence of organisms, including identification of bacteria.
Results: A total of 318 of the 330 forceps were evaluable; 314 forceps (98.74 %) were sterile after use once, five times, or 20 times. Four forceps were contaminated with Staphylococcus epidermidis (n = 2), Bacillus licheniformis (n = 1) and Corynebacterium aquaticum (n = 2). All of 25 forceps were sterile after being used 20 times.
Conclusion: Colonoscopy biopsy forceps can be reliably reprocessed following this standardized protocol, even without aldehydes.

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M. Jung, M.D.

Dept. of Internal Medicine · St. Hildegardis Hospital

Hildegardstrasse 2 · 55131 Mainz · Germany

Fax: + 49-6131-147753

Email: M.Jung.Hildegardis-Mz@t-online.de