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DOI: 10.1055/s-2002-35661
© Georg Thieme Verlag Stuttgart · New York
In Vivo Antioxidant Action of a Lignan-Enriched Extract of Schisandra Fruit and an Anthraquinone-Containing Extract of Polygonum Root in Comparison with Schisandrin B and Emodin
Publikationsverlauf
Received: February 13, 2002
Accepted: June 8, 2002
Publikationsdatum:
26. November 2002 (online)
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Abstract
The in vivo antioxidant action of a lignan-enriched extract of the fruit of Schisandra chinensis (FS) and an anthraquinone-containing extract of the root of Polygonum multiflorum (PME) was compared with their respective active constituents schisandrin B (Sch B) and emodin by examining their effect on hepatic mitochondrial glutathione antioxidant status in control and carbon tetrachloride (CCl4)-intoxicated mice. FS and PME pretreatments produced a dose-dependent protection against CCl4 hepatotoxicity, with the effect of FS being more potent. Pretreatment with Sch B, emodin or α-tocopherol (α-Toc) also protected against CCl4 hepatotoxicity, with the effect of Sch B being more potent. The extent of hepatoprotection afforded by FS/Sch B and PME/emodin pretreatment against CCl4 toxicity was found to correlate well with the degree of enhancement in hepatic mitochondrial glutathione antioxidant status, as evidenced by increases in reduced glutathione level and activities of glutathione reductase, glutathione peroxidase as well as glutathione S-transferases, in both control and CCl4-intoxicated mice. α-Toc, which did not enhance mitochondrial glutathione antioxidant status, seemed to be less potent in protecting against CCl4 hepatotoxicity. The ensemble of results indicates that FS/PME produced a more potent in vivo antioxidant action than α-Toc by virtue of their ability to enhance hepatic mitochondrial glutathione antioxidant status and that the differential potency of FS and PME can be attributed to the difference in in vivo antioxidant potential between Sch B and emodin.
Abbreviations
ALT:alanine aminotransferases
CCl4:carbon tetrachloride
FS:lignan-enriched extract of Schisandra fruit
GRD:glutathione reductase
GSH:reduced glutathione
GSH-Px:Se-glutathione peroxidase
GST:glutathione S-transferases
mt:mitochondrial
MDA:malondialdehyde
PME:anthraquinone-containing fraction of Polygonum root
Sch B:schisandrin B
SDH:sorbitol dehydrogenase
α-Toc:α-tocopherol
Key words
Polygonum multiflorum - Polygonaceae - Schisandra chinensis - Schisandraceae - mitochondrion - glutathione - α-tocopherol
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Dr. Robert Ko
Department of Biochemistry
Hong Kong University of Science & Technology
Clear Water Bay, Hong Kong, China
eMail: bcrko@ust.hk
Fax: (852) 2358 1552