Planta Med 2002; 68(6): 505-509
DOI: 10.1055/s-2002-32564
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Inhibition of Cultured Bovine Aortic Endothelial Cell Proliferation by Sodium Spirulan, A New Sulfated Polysaccharide Isolated from Spirulina platensis

Toshiyuki Kaji1 , Yasuyuki Fujiwara1 , Chieko Hamada1 , Chika Yamamoto1 , Satomi Shimada1 , Jung-Bum Lee2 , Toshimitsu Hayashi2
  • 1Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan
  • 2Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama, Japan
Weitere Informationen

Publikationsverlauf

August 30, 2001

February 2, 2002

Publikationsdatum:
01. Juli 2002 (online)

Abstract

Sodium spirulan (Na-SP) is a sulfated polysaccharide isolated from the blue-green alga Spirulina platensis, which consists of two types of disaccharide repeating units, O-hexuronosyl-rhamnose (aldobiuronic acid) and O-rhamnosyl-3-O-methylrhamnose (acofriose) with sulfate groups, other minor saccharides and sodium ion. Vascular endothelial cells are present on the inner surface of blood vessels in a monolayer and have anticoagulant properties. To address the question whether Na-SP influences the maintenance of endothelial cell monolayers, we investigated the proliferation of cultured bovine aortic endothelial cells treated with Na-SP. It was found that Na-SP has an inhibitory activity on endothelial cell proliferation accompanied with suppression of whole protein synthesis but without non-specific cell damage. The inhibitory activity of Na-SP was the strongest when compared to that of heparan sulfate, heparin, dextran sulfate, dermatan sulfate, chondroitin sulfate A/C and hyaluronan. Furthermore, it was shown that the inhibitory activity of Na-SP disappeared by either desulfation or depolymerization. The present data suggest that Na-SP is a unique sulfated polysaccharide that strongly inhibits vascular endothelial cell proliferation, and the inhibitory activity requires polymerization of sulfated O-rhamnosyl-acofriose repeating units.

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Dr. Toshiyuki Kaji

Department of Environmental Health

Faculty of Pharmaceutical Sciences


Hokuriku University

Ho-3 Kanagawa-machi

Kanazawa 920-1181

Japan

eMail: t-kaji@hokuriku-u.ac.jp

Fax: +81-76-229-6208