Planta Med 2002; 68(6): 492-496
DOI: 10.1055/s-2002-32563
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effects of Glycosides from the Leaves of Melaleuca quinquenervia on Vascular Contraction of Rats

Tzong-Huei Lee1 , Guei-Jane Wang2 , Ching-Kuo Lee3 , Yueh-Hsiung Kuo4 , Chang-Hung Chou1, 5
  • 1Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan
  • 2National Research Institute of Chinese Medicine, Shih-Pai, Taipei, Taiwan
  • 3China Junior College of Medical Technology, Tainan, Taiwan
  • 4Department of Chemistry, National Taiwan University, Taipei, Taiwan
  • 5Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan
Further Information

Publication History

August 16, 2001

January 20, 2002

Publication Date:
01 July 2002 (online)

Abstract

Two new glycosides, 3-hydroxy-5-methoxy-4-methylphenyl β-D-glucopyranoside (1) and 4-benzoyl-2-C-β-glucopyranosyl-3,5-dihydroxy-6-methylphenyl β-D-glucopyranoside (2), together with four known glycosides, 2-endo-β-D-glucopyranosyloxy-1,8-cineole (3a), 2-exo-β-D-glucopyranosyloxy-1,8-cineole (3b), roseoside (4), and citroside A (5), were isolated from the methanolic extract of leaves of Melaleuca quinquenervia. Their structures were elucidated on the basis of spectroscopic analysis. Compounds 1, 2a and 3 inhibited contractile response induced by phenylephrine in aortic rings from Sprague-Dawley rats. This inhibition was independent of the endothelium. Compounds 2 and 4 significantly relaxed precontracted aortic rings, in an endothelium-dependent manner. Pretreatment of N ω-nitro-L-arginine (L-NNA), a nitric oxide synthase inhibitor, partially attenuated the vasorelaxation induced by both compounds, suggesting that nitric oxide was likely the responsible mediator. The rank-order potency (EC50 value) of vasorelaxing activities of these compounds is 4 > 2 > 2a > 3 > 1.

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Prof. Chang-Hung Chou

Department of Biological Sciences

National Sun Yat-sen University

Kaohsiung

Taiwan 804 R.O.C.

Phone: +886 2 26510363

Fax: +886 2 26510363

Email: choumasa@mail.nsysu.edu.tw