Planta Med 2002; 68(6): 574-576
DOI: 10.1055/s-2002-32559
Letter
© Georg Thieme Verlag Stuttgart · New York

Studies on the Molecular Markers of Rhizomes of some Alpinia Species

Z. L. Zhao1 , Z. T. Wang2 , L. S. Xu2 , K. Y. Zhou3
  • 1Chongqing Academy of Chinese Materia Medica, Chongqing, P.R. China
  • 2College of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, P.R.China
  • 3Nanjing Normal University, Nanjing, P.R. China
Further Information

Publication History

September 29, 2001

January 12, 2002

Publication Date:
01 July 2002 (online)

Abstract

The rhizomes of Alpinia jianganfeng are used as a traditional Chinese medicine, Jian Gan Feng, to cure rheumatism in Guangdong, China. The rhizomes of some other species of the genus Alpinia such as A. japonica, A. suishaensis and A. nanchuanensis are also used as Jian Gan Feng in Southwest China. However, the identification of the original plants of the crude drugs is difficult. The internal transcribed spacers and the 5.8S coding region of nuclear ribosomal DNA of the four species were sequenced and analyzed. The DNA markers have been determined and they can be used for the molecular identification of these medicinal plants.

References

  • 1 Xie Z W. Quan Guo Zhong Cao Yao Hui Bian. Vol. 2 People’s Medical Publishing House 1996: 677-8
  • 2 Jiangsu New Medical College . Zhong Yao Da Ci Dian. Vol. 1 Shanghai People’s Publishing House 1977: 169-70
  • 3 Sichuan Institute of Chinese Materia Medica . Common Chinese Herbs in Sichuan Province. Sichuan People’s Publishing House 1971: 1345-6
  • 4 Suh Y, Thien L B, Reeve H E, Zimmer E A. Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA in Winteraceae.  American Journal of Botany. 1993;  80 1042-55
  • 5 Fu R Z, Wang J, Zhang Y B, Wang Z T, But P PH, Li N, Shaw P C. Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism.  Planta Medica. 1999;  65 48-50
  • 6 Zhang Y B, Ngan F N, Wang Z T, Ng T B, But P PH, Shaw P C, Wang J. Random primed polymerase chain reaction differentiates Codonopsis pilosula from different localities.  Planta Medica. 1999;  65 157-60
  • 7 Cai Z H, Li P, Dong T TX, Tsim K WK. Molecular diversity of 5S-rRNA spacer domain in Fritillaria species revealed by PCR analysis.  Planta Medica. 1999;  65 360-4
  • 8 Zhao Z L, Zhou K Y, Wang Z T, Qin M J, Dong H, Xu L S. Determination of nrDNA ITS sequences of Caodoukou (Alpinia hainanensis K. Schum.) and Yizhi (A. oxyphylla Miq.)  Journal of Plant Resources and Environment. 2000;  9 38-40
  • 9 Zhao Z L, Zhou K Y, Dong H, Xu L S. Characters of nrDNA ITS region sequences of fruits of Alpinia galanga and their adulterants.  Planta Medica. 2001;  67 381-3
  • 10 Baldwin B G, Sanderson M J, Porter J M, Wojciechowski M F, Campbell C S, Donoghue M J. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny.  Annals of the Missouri Botanical Garden. 1995;  82 247-77

Professor Zhou Kaiya

Jiangsu Key Laboratory of Resources Biotechnology

Nanjing Normal University

Nanjing 210097

China

Email: kyzhounj@jlonline.com

Fax: +86-25-3598328