Horm Metab Res 2002; 34(5): 260-264
DOI: 10.1055/s-2002-32140
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Non-Enzymatic Glycation of IgG: An In Vivo Study

A.  Lapolla 1 , R.  Tonani 2 , D.  Fedele 1 , M.  Garbeglio 1 , A.  Senesi 1 , R.  Seraglia 3 , D.  Favretto 3 , P.  Traldi 3
  • 1Dipartimento Scienze Mediche e Chirurgiche, Università di Padova, Padova, Italy
  • 2Pharmacia, Nerviano, Milano, Italy
  • 3CNR, Padova, Italy
Further Information

Publication History

7 January 2002

7 Febrary 2002

Publication Date:
10 June 2002 (online)

Abstract

The IgG glycation level of 30 healthy subjects and 60 type 2 diabetic patients with different degrees of metabolic control was evaluated by matrix-assisted laser desorption ionization mass spectrometry, a technique allowing the determination of mass increase of the IgG molecule. When applied to the digested mixture obtained by the action of papain on the plasma protein fraction, the same method established the mass increase of Fab and Fc fragments of IgG; for the former, a higher mass increase was found, possibly explained by its high reactivity to glucose. Experimental results were confirmed by molecular modeling calculations. Results suggest that the immunodeficiency observed in diabetic patients may be due to the inhibition of molecular recognition between antibody and antigen as a result of a change in functionality of the modified Fab fragment of IgG.

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A. Lapolla

Cattedra Malattie del Metabolismo · Dipartimento Scienze Mediche e Chirurgiche ·

Università di Padova · Via Giustiniani 2 · 35100 Padova · Italy ·

Phone: + 39 (49) 821 62 68 ·

Fax: + 39 (49) 821 62 66

Email: annunziata.lapollo-@unipd.it