RSS-Feed abonnieren
DOI: 10.1055/s-2002-30185
Vascularized Bone-Marrow Allotransplantation in Rats Prolongs the Survival of Simultaneously Grafted Alloskin
Publikationsverlauf
Publikationsdatum:
17. Mai 2002 (online)
![](https://www.thieme-connect.de/media/jrm/200204/lookinside/thumbnails/10.1055-s-2002-30185-1.jpg)
ABSTRACT
Skin allografts in rats were rejected, even if they were treated with a sub-optimal dose of FK506. However, skin allografts in rats which simultaneously received vascularized limb allografts were rejected more slowly. In this study, the authors examined the reasons why vascularized limb allografts have the ability to induce immunologic tolerance. For investigating which tissue components provide such tolerance, they compared the mean survival times (MST) of skin allografts on the backs of rats (used as an indicator of rejection), which were simultaneously engrafted with vascularized skin allografts, muscle allografts, joint allografts, and limb allografts. In the cases of limb allografts (MST=32.1 days) (these allografts contain bone-marrow cells), the skin allografts survived longer than vascularized skin allografts (MST=23.6 days) or muscle allografts (MST=22.6 days). For ascertaining whether the ability to induce immunologic tolerance of limb allografts was due to bone-marrow cells or not, the authors compared the MST of skin allografts in rats that were simultaneously engrafted with non-irradiated limb allografts, irradiated limb allografts, and bone-marrow cells. The allografted skin in the rats that received non-irradiated limbs (MST=32.1 days) survived longer than irradiated limb allografts (MST=20.3 days) and bone-marrow cells (MST=18.4 days). Limb allografts are, as it were, vascularized bone-marrow allografts. These results suggested that vascularized bone-marrow allografts can facilitate the induction of immunologic tolerance.
KEYWORDS
Bone-marrow allotransplantation - induction of immunologic tolerance - experimental
REFERENCES
- 1 Hewitt C W, Black K S, Stenger J A. Comparison of kidney, composite tissue, and skin allograft survival in rats prolonged by donor blood and concomitant limited cyclosporine. Transplant Proc . 1988; 20 1110
- 2 Inamura N, Nakahara K, Kino T. Prolongation of skin allograft survival in rats by a novel immunosuppressive agent, FK506. Transplantation . 1988; 45 206
- 3 Achauer B M, Hewitt C W, Black K S. Cyclosporine prolongs skin allografts in rat burn model. Transplant Proc . 1983; 15 3073
- 4 Towpik E, Kupiec-Weglinski J W, Tyler D S. Cyclosporine and experimetal skin allografts: long term survival in rats treated with low maintenance doses. Plast Reconstr Surg . 1986; 77 268
- 5 Lim S ML, Li S Q. Induction of tolerance to vascularized skin allografts using cyclosporine A-an experimental study on rats. Ann Acad Med . 1991; 20 484
- 6 Watt D J, Patridge T A, Sloper J C. Cyclosporine A as a means of preventing rejection of skeletal muscle allografts in mice. Transplantation . 1981; 31 266
- 7 Tan C M, Yaremchuk M J, Randolph M A. Vascularized muscle allografts and the role of cyclosporine. Plast Reconst Surg . 1991; 87 412
- 8 Paskert J P, Yaremchuk M J, Randolph M A, Weiland A J. The role of cyclosporine in prolonged survival in vascularized bone allografts. Plast Reconstr Surg . 1987; 80 240
- 9 Black K S, Hewitt C W, Fraser L A. Composite tissue (limb) allografts in rats. II. Indefinite survival using low-dose cyclosporine. Transplantation . 1985; 39 365
- 10 Arai K, Hotokebuchi T, Miyahara H. Limb allografts in rats immunosuppressed with FK506. I. Reversal of rejection and indefinite survival. Transplantation . 1989; 48 782
- 11 Sakai K, Hickey M J, Hurley J V. Vascularised ostechondral allografts in an immunosuppressed rat model: graft modulation and host immune tolerance. Plast Reconstr Surg . 1993; 91 597
- 12 Lee W P, Yaremchuk M J, Pan Y C. Relative antigenicity of components of a vascularized limb allograft. Plast Reconstr Surg . 1991; 87 401
- 13 Lee W P, Randolph M A, Weiland A J, Yaremchuk M J. Prolonged survival of vascularized limb tissue allografts by donor irradiation. J Surg Res . 1995; 59 578
- 14 Talmor M, Steinman R M, Codner M A. Bone marrow-derived chimerism in non-irradiated, Cyclosporine-treated rats receiving microvascularized limb transplants: evidence for donor-derived dendritic cells in recipient lymphoid tissues. Immunology . 1995; 86 448
- 15 Okazaki H, Maki T, Wood M L. Prolongation of skin allograft survival in H-2 K and I region-incompatible mice by pretransplant blood transfusion. Transplantation . 1981; 32 111
- 16 Opelz G, Graver B, Terasaki P. Induction of high kidney graft survival rate by multiple transfusion. Lancet . 1981; 1 1223
- 17 Llull R, Murase N, Ye Q. Vascularized bone marrow transplantation in rats: evidence for amplification of hematolymphoid chimerism and freedom from graft-versus-host reaction. Transplant Proc . 1995; 27 164
- 18 Starzl T E, Demetris A J, Murase N. Cell migration, chimerism, and graft acceptance. Lancet . 1992; 339 1579