Planta Med 2002; 68(4): 316-321
DOI: 10.1055/s-2002-26742
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Effects of Ginkgetin from Ginkgo biloba Leaves on Cyclooxygenases and In Vivo Skin Inflammation

Wie-Jong Kwak1 , Chang Kyun Han1 , Kun Ho Son2 , Hyeun Wook Chang3 , Sam Sik Kang4 , Byoung Kyu Park5 , Hyun Pyo Kim5
  • 1SK Chemicals Ltd., Suwon, Korea
  • 2Dept. Food Nutr., Andong National University, Andong, Korea
  • 3College of Pharmacy, Yeoungnam University, Kyoungsan, Korea
  • 4Nat. Products Res. Inst., Seoul National University, Seoul, Korea
  • 5College of Pharmacy, Kangwon National University, Chunchon, Korea
Further Information

Publication History

June 21, 2001

August 25, 2001

Publication Date:
02 May 2002 (online)

Abstract

Ginkgetin, a biflavone from Ginkgo biloba leaves, was previously reported to be a phospholipase A2 inhibitor and this compound showed the potent antiarthritic activity in rat adjuvant-induced arthritis as well as analgesic activity. This investigation was carried out to find effects on cyclooxygenase (COX)-1 and -2 including an in vivo effect. Ginkgetin (1 - 10 μM) and the biflavonoid mixture (10 - 50 μg/ml), mainly a 1 : 1 mixture of ginkgetin and isoginkgetin, from G. biloba leaves, inhibited production of prostaglandin E2 from lipopolysaccharide-induced RAW 264.7 cells. This inhibition was mediated, at least in part, by down-regulation of COX-2 expression, but not by direct inhibition of COX-1 or COX-2 activity. Down-regulation of COX-2 by ginkgetin was also proved in the dorsal skin of ICR mouse treated by 12-O-tetradecanoylphorbol 13-acetate (TPA). At total doses of 1,000 μg/site on the dorsal skin (15 mm × 15 mm), ginkgetin inhibited prostaglandin E2 production by 65.6 % along with a marked suppression of COX-2 induction. In addition, ginkgetin and the biflavonoid mixture (100 - 1,000 μg/ear) dose-dependently inhibited skin inflammation of croton oil induced ear edema in mice by topical application. The present study suggests that ginkgetin from G. biloba leaves down-regulates COX-2 induction in vivo and this down-regulating potential is associated with an anti-inflammatory activity against skin inflammatory responses.

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Prof. Dr. H. P. Kim

College of Pharmacy

Kangwon National University

Chunchon 200-701

Republic of Korea

Email: hpkim@cc.kangwon.ac.kr

Fax: +82-33-255-9271